Anti-ACE2 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9BYF1

Core Product Specifications and Parameters

Biological Background: ACE2 Function and Localization

• ACE2 (Angiotensin-Converting Enzyme 2), also known as ACEH, is a carboxypeptidase that critically regulates the renin-angiotensin system (RAS) by converting Angiotensin II to the vasodilator Angiotensin 1-7, and Angiotensin I to Angiotensin 1-9. Related references: PMID:10924499 PMID:10969042 PMID:11815627
• It also cleaves other vasoactive peptides including neurotensin, kinetensin, des-Arg bradykinin, apelins, casomorphins, and dynorphin A, acting as a counter-regulatory enzyme to ACE. Related references: PMID:10969042 PMID:11815627 PMID:27217402 PMID:28293165
• ACE2 serves as the cellular receptor for SARS-CoV and SARS-CoV-2, facilitating viral entry.
• Localized to the cell membrane, particularly the apical membrane of epithelial cells, as well as in the cytoplasm and secreted forms.
• Expressed in heart, kidney, testis, and gastrointestinal tract; low expression in lung alveolar type 2 cells, nasal and bronchial epithelial cells.
• ACE2 gene produces multiple isoforms through alternative splicing.
• Keywords: carboxypeptidase, metalloprotease, host cell receptor for virus entry, zinc-binding, glycoprotein, phosphoprotein (UniProt Q9BYF1).

Experimental Guidance and Technical Tips

• The immunogen covers a large portion of the extracellular domain (aa 18–740), potentially allowing recognition of both native and denatured ACE2.
• For Western blot under reducing conditions, a band around 92 kDa is expected; validate with positive controls such as kidney or heart lysates.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods and validate on kidney or intestinal tissue sections.
• For immunofluorescence (IF-P), permeabilization may be necessary for intracellular epitope access; co-localization with membrane markers is advised.
• Cross-reactivity with mouse and rat ACE2 is listed; verify specificity via sequence alignment and using appropriate controls.
• In SARS-CoV-2 research, spike protein competition assays can confirm specificity for the receptor-binding domain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories by providing validated polyclonal antibodies like this anti-ACE2 antibody, alongside comprehensive raw material sourcing for IVD development. Whether you need matched antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, or assistance with rare target reagents, CamelBio streamlines procurement from concept to clinic. Contact us to explore how we can accelerate your ACE2-related research or assay development project.