Anti-ACE2 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9BYF1

Core Product Specifications and Parameters

Biological Background: ACE2 Function and Localization

• ACE2 is a zinc metalloprotease carboxypeptidase that critically regulates the renin-angiotensin system by converting angiotensin II to angiotensin 1-7, acting as a vasodilator and counterbalancing the effects of angiotensin II vasoconstriction. Related references: PMID:27217402, PMID:10924499, PMID:10969042, PMID:11815627
• It also converts angiotensin I to angiotensin 1-9 and processes other vasoactive peptides (neurotensin, kinetensin, des-Arg bradykinin) and biological peptides (apelins, casomorphins, dynorphin A) with high efficiency. Related references: PMID:10969042, PMID:11815627, PMID:27217402, PMID:28293165
• The C-terminal collectrin homology domain facilitates trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane, regulating its expression and catalytic activity. Related references: PMID:18424768, PMID:19185582
• ACE2 serves as the entry receptor for human coronaviruses SARS-CoV, SARS-CoV-2, and HCoV-NL63, playing a pivotal role in viral pathogenesis.
• Subcellularly, ACE2 localizes to the plasma membrane (apical in polarized cells), cytoplasm, secreted fraction, and cilia of cell projections.
• ACE2 is widely expressed in endothelial cells, arterial smooth muscle, renal proximal tubules, small intestine enterocytes, heart, testis, and gastrointestinal tract, with highly variable and generally low expression in lung alveolar type 2 cells. Related references: PMID:15141377, PMID:18424768, PMID:10924499, PMID:10969042, PMID:12459472, PMID:15231706, PMID:15671045, PMID:32170560, PMID:32715618, PMID:32425701, PMID:33432184, PMID:32333915, PMID:32327758, PMID:32358202, PMID:32413319, PMID:32404436
• Post-translational modifications of ACE2 include glycosylation, phosphorylation, and ubiquitination, which may impact its stability and function.

Experimental Guidance and Technical Tips

• The immunogen spans residues 18–740 of human ACE2, encompassing the entire extracellular domain. This design is expected to detect full-length endogenous ACE2 across multiple applications.
• For Western blot, a specific band near 92 kDa should be observed under reducing conditions. Use validated positive control lysates from human kidney, heart, or intestinal tissue, or from mouse/rat tissues given reported cross-reactivity.
• For immunohistochemistry (paraffin-embedded) and immunofluorescence, antigen retrieval is often required. Kidney proximal tubules, small intestine enterocytes, and testis serve as strong positive controls due to high ACE2 expression.
• In ELISA assays, this antibody may function as capture or detection reagent. Titration and pairing with a second ACE2 antibody clone are recommended for optimal sandwich ELISA performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic developers and researchers with bulk monoclonal antibodies, ancillary reagents, and technical consultation. For ACE2-related research—particularly in cardiovascular disease, renin-angiotensin system studies, and coronavirus receptor biology—we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, and access to rare-target raw materials. Contact us to streamline your supply chain from concept to clinic.