Anti-ACE1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P12821

Core Product Specifications and Parameters

Biological Background: ACE1 Function and Localization

• ACE1 is a dipeptidyl carboxypeptidase that removes C-terminal dipeptides from circulating hormones like angiotensin I and bradykinin, playing a key role in blood pressure regulation and electrolyte homeostasis. Related references: PMID:15615692 PMID:20826823 PMID:2558109
• ACE1 converts angiotensin I to the potent vasoconstrictor angiotensin II and inactivates the vasodilator bradykinin, central to the renin-angiotensin system. Related references: PMID:11432860 PMID:1851160 PMID:19773553 PMID:15615692 PMID:2558109 PMID:4322742
• The enzyme mediates cleavage of neuropeptide hormones, such as substance P and enkephalins, modulating synaptic transmission and plasticity. Related references: PMID:15615692 PMID:6208535 PMID:6270633 PMID:2982830 PMID:656131
• ACE1 contains two homologous catalytic domains (N- and C-terminal) with distinct substrate specificities; the N-domain degrades AcSDKP and amyloid-beta peptides, while the C-domain cleaves angiotensin I and cholecystokinin. Related references: PMID:10913258 PMID:1320019 PMID:1851160 PMID:10336644 PMID:19773553 PMID:7876104
• A testis-specific isoform with a single catalytic domain is produced by alternative promoter usage and is essential for male fertility. Related references: PMID:1651327 PMID:1668266 PMID:24297181
• Ubiquitously expressed with highest levels in lung, kidney, heart, gastrointestinal tract, and prostate; a testis-specific isoform is found in spermatocytes and adult testis.
• Subcellular localization includes cell membrane, cytoplasm, and secreted forms; the protein undergoes proteolytic cleavage to release a soluble ectodomain.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–1261 of human ACE1, covering nearly the full-length somatic ectodomain; the antibody likely recognizes both somatic and testis isoforms.
• For WB, use standard SDS-PAGE under reducing conditions; the expected molecular weight for the somatic isoform is ~150 kDa, while the testis-specific isoform may appear at ~100 kDa. Validate in appropriate tissue lysates (lung, kidney, testis).
• Due to the large immunogen, the antibody may detect conformational epitopes; consider native or mild denaturing conditions for immunofluorescence (IF-P) and immunohistochemistry (IHC-P).
• For ELISA, the antibody can be used for direct or sandwich formats; optimize coating antigen and detection conditions using recombinant ACE1 protein.
• Cross-reactivity with mouse and rat ACE1 has been reported; verify species-specific performance using tissue controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. For ACE1-related cardiovascular, neuropeptide, or fertility research, CamelBio provides high-quality reagents to support your diagnostic development from concept to clinic. Contact us to discuss your specific reagent needs and supply requirements.