Anti-ACD Rabbit Polyclonal Antibody for WB and ELISA - Q96AP0

Core Product Specifications and Parameters

Biological Background: Adrenocortical dysplasia protein homolog (ACD) Function and Localization

• ACD (Adrenocortical dysplasia protein homolog), also known as POT1 and TIN2-interacting protein, is a component of the shelterin (telosome) complex that protects chromosome ends by binding to double-stranded TTAGGG repeats.
• It promotes the binding of POT1 to single-stranded telomeric DNA, modulating the inhibitory effects of POT1 on telomere elongation.
• The ACD-POT1 heterodimer enhances telomere elongation by recruiting telomerase to telomeres and increasing its processivity.
• This protein localizes to the nucleus and is specifically associated with chromosome telomeres.
• Alternative splicing produces multiple isoforms, and post-translational modifications include phosphorylation and ubiquitin-like conjugation (isopeptide bond).
• Disease variants of ACD are associated with dyskeratosis congenita, highlighting its critical role in telomere biology.
• It may also play a role in organogenesis.
• The shelterin complex, of which ACD is a part, prevents chromosome ends from being recognized as DNA damage, thereby maintaining genomic stability.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (aa 1-220) of human ACD. This region may contain functional domains important for interactions; the resulting polyclonal antibody may recognize multiple epitopes.
• For Western blotting, a band corresponding to the predicted molecular weight of approximately 49 kDa (calculated 48.9 kDa) may be observed in human cell lysates. Consider optimizing lysis and loading conditions to detect endogenous ACD.
• In ELISA applications, this antibody may be suitable for detecting recombinant or overexpressed ACD; appropriate controls and titration are recommended.
• As cross-reactivity is indicated only for human, validation in other species should be performed before use in non-human systems.
• Due to the nuclear and telomere-specific localization of ACD, consider appropriate sample preparation methods to enrich for nuclear extracts or telomere-associated proteins if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic developers from concept to clinic. For telomere biology research and assay development, we provide validated antibodies, antibody pairs, and bulk ancillary reagents tailored to your needs. Our expertise in rare target sourcing ensures access to critical tools for exploring shelterin complex components and their roles in disease.