Anti-ACBD3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H3P7

Core Product Specifications and Parameters

Biological Background: ACBD3 Function and Localization

• ACBD3 (Acyl-CoA-binding domain-containing protein 3), also known as GCP60, GOCAP1, and GOLPH1, is a Golgi-resident protein involved in maintaining Golgi structure and membrane traffic. Related references: PMID:11590181
• Through interaction with giantin, ACBD3 influences protein transport between the endoplasmic reticulum and the Golgi apparatus (PubMed:11590181).
• ACBD3 plays a key role in hormone-induced steroid biosynthesis in testicular Leydig cells (by similarity).
• It recruits phosphatidylinositol 4-kinase beta (PI4KB) to Golgi membranes, enhancing PI4KB activity and increasing local substrate concentration for phosphoinositide synthesis (PubMed:27009356). Related references: PMID:27009356
• During Aichi virus infection, ACBD3 is essential for viral RNA replication by recruiting PI4KB to viral replication sites.
• Subcellularly, ACBD3 localizes to the Golgi apparatus membrane and mitochondria.
• Ubiquitously expressed, with highest levels in testis and ovary, consistent with its role in steroidogenic tissues.
• Post-translational modifications include phosphorylation and acetylation; the protein contains a coiled-coil domain.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–160 of human ACBD3, targeting the N-terminal region. This region may detect all reported isoforms.
• For Western blot, an expected band around 61 kDa (predicted MW ~60.6 kDa) should be observed in human, mouse, and rat samples; consider using positive controls like testis or ovary lysates.
• In immunofluorescence, ACBD3 localizes to the Golgi apparatus and mitochondria; appropriate fixation and permeabilization (e.g., methanol or Triton X-100) are recommended to visualize these membranous structures.
• For ELISA applications, confirm antigen coating and detection conditions empirically using recombinant ACBD3 protein.
• Validate specificity with siRNA knockdown or CRISPR knockout lysates to confirm band disappearance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a single access point for IVD raw materials, technical services, and consulting—from concept to clinic. This ACBD3 antibody supports research into Golgi biology, steroidogenesis, and host-virus interactions. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can assist with rare target sourcing to streamline your development workflow.