Anti-ACAT2 Rabbit Monoclonal Antibody for WB, ELISA - Q9BWD1

Core Product Specifications and Parameters

Biological Background: ACAT2 Function and Localization

• ACAT2 encodes cytosolic acetyl-CoA acetyltransferase, an enzyme that catalyzes the formation of acetoacetyl-CoA from two acetyl-CoA molecules, a key step in cholesterol biosynthesis.
• Also known as acetoacetyl-CoA thiolase or acetyl-CoA transferase-like protein, ACAT2 is primarily expressed in the liver and intestine, tissues with high cholesterol synthesis activity.
• The enzyme functions as a homotetramer in the cytosol, consistent with its subcellular localization in the cytoplasm.
• Human ACAT2 shares homology with mitochondrial ACAT1 but differs in subcellular targeting and tissue distribution, highlighting its specialized role in cytosolic lipid metabolism.
• Alternative splicing produces multiple isoforms of ACAT2, which may regulate enzymatic activity or protein interactions.
• Post-translational modifications, including acetylation, have been identified by proteomics, potentially affecting enzyme stability or activity.
• Structural studies (3D-structure available) reveal the active-site architecture and mechanism of acyl transfer, supporting its classification as a transferase.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 41 kDa (calculated 41.351 kDa), so use appropriate gel percentage and transfer conditions.
• The synthetic peptide immunogen (aa 150‑250) may react with specific isoforms; validate target detection in relevant cell or tissue lysates.
• Cross-reactivity with mouse and rat ACAT2 has been reported; however, perform species-specific validation in your experimental system.
• ELISA applications may require optimization of coating antigen and antibody concentration; consider direct or sandwich formats.
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency, suitable for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research labs with rapid access to validated antibodies, bulk ancillary reagents, and rare-target procurement support. This ACAT2 antibody, targeting cholesterol biosynthesis, exemplifies our portfolio of high-quality reagents for metabolic and cardiovascular disease research. Whether you need optimized monoclonal antibodies, validated antibody pairs, or customized sourcing solutions, CamelBio streamlines your journey from concept to clinic.