Anti-ACAT1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24752

Core Product Specifications and Parameters

Biological Background: ACAT1 Function and Localization

• ACAT1 is a mitochondrial acetyl-CoA acetyltransferase, also known as acetoacetyl-CoA thiolase or T2.
• As a key enzyme in the mitochondrial β-oxidation pathway, it catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a shortened fatty acyl-CoA, essential for fatty acid degradation. Related references: PMID:1715688, PMID:7728148, PMID:9744475
• The enzyme also functions reversibly, condensing two acetyl-CoA molecules into acetoacetyl-CoA, playing a major role in ketone body metabolism. Related references: PMID:17371050
• ACAT1 localizes to the mitochondrion, consistent with its role in lipid metabolism.
• Post-translational modifications include acetylation; the protein is also subject to alternative splicing and has disease variant associations.
• Additional keywords: acyltransferase, metal-binding (potassium), transferase, transit peptide.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–145) of human ACAT1. Consider this when selecting detection antibodies for assays involving N-terminal modifications or cleavage products.
• For WB, a band at approximately 45 kDa (full-length) may be observed; validate in the relevant sample system to confirm specificity.
• For IHC-P, consider that ACAT1 is mitochondrial; appropriate antigen retrieval and permeabilization may enhance detection.
• ELISA can be used for quantifying ACAT1 in biological samples; ensure standard curves are generated with recombinant ACAT1 of known concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects focused on mitochondrial β-oxidation or ketone body metabolism, we offer one-stop sourcing support from concept to clinic, helping you secure the critical reagents needed for your assays.