Anti-ACAT1 Rabbit Polyclonal Antibody for WB and ELISA - P24752

Core Product Specifications and Parameters

Biological Background: Acetyl-CoA acetyltransferase, mitochondrial Function and Localization

• Acetyl-CoA acetyltransferase, mitochondrial (EC 2.3.1.9), also known as acetoacetyl-CoA thiolase, T2, MAT, ACAT, or THIL, is encoded by the ACAT1 gene (Gene ID: 38) in humans.
• It catalyzes the last step of the mitochondrial beta-oxidation pathway, thiolytically cleaving medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a shortened acyl-CoA (PubMed:1715688, PubMed:7728148, PubMed:9744475).
  Related references: PMID:1715688 PMID:7728148 PMID:9744475
• The enzyme reaction is reversible: it can condense two acetyl-CoA molecules into acetoacetyl-CoA, playing a central role in ketone body metabolism (PubMed:17371050, PubMed:1715688, PubMed:7728148).
  Related references: PMID:17371050 PMID:1715688 PMID:7728148
• It localizes to the mitochondrion and contains a transit peptide required for mitochondrial import.
• The protein is subject to acetylation and alternative splicing, and its activity may be influenced by potassium ion binding.
• Mutations in ACAT1 are associated with beta-ketothiolase deficiency, a rare metabolic disorder (Keywords: Disease variant).
• The calculated molecular weight is 45.2 kDa, with mature protein observed at ~45 kDa.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–145 of human ACAT1 (NP_000010.1), covering the N-terminal region which includes the mitochondrial transit peptide and part of the mature protein. This design may favor detection of precursor and mature forms.
• Recommended for western blot (WB) and ELISA applications. For WB, a predominant band near 45 kDa is expected in human, mouse, and rat lysates.
• As a polyclonal antibody, potential cross-reactivity with homologous family members or ACAT1 isoforms from alternative splicing should be considered; validation in the relevant sample matrix is advised.
• For ELISA, consider using recombinant ACAT1 or mitochondrial-enriched protein extracts as coating antigen, and include appropriate controls for specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-ACAT1 antibody addresses research needs in mitochondrial fatty acid beta-oxidation, ketone body metabolism, and related metabolic disorders. Our global sourcing network can also assist with rare targets and custom assay development—contact us to discuss your project requirements.