Anti-ACAP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15057

Core Product Specifications and Parameters

Biological Background: ACAP2 Function and Localization

• ACAP2 (Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2), also known as Centaurin-beta-2 (Cnt-b2), is a multidomain GTPase-activating protein.
• It functions as a GTPase-activating protein (GAP) for ADP-ribosylation factor 6 (ARF6), but does not show GAP activity for RAB35. Related references: PMID:30905672
• Contains ANK repeats, coiled-coil regions, a PH domain, and a zinc-finger motif, which mediate protein–protein interactions and membrane targeting.
• Localizes to the cell membrane and endosome membrane, consistent with its role in endosomal trafficking and remodeling of the actin cytoskeleton.
• Widely expressed in human tissues, with the highest expression level observed in the lung.
• Post-translational modifications include phosphorylation (phosphoprotein), and metal-binding via its zinc-finger domains.
• Has been identified in proteomics screens, underscoring its abundance and potential roles in diverse signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–160 of human ACAP2 (NP_036419.3), which lies within the N-terminal region likely containing part of the coiled-coil or ANK repeat domains; consider this when mapping epitopes.
• For western blot (WB) analysis, the predicted molecular weight is 88 kDa; ensure adequate separation and transfer conditions for proteins of this size.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect membrane and endosomal staining patterns; validate specificity using appropriate controls.
• This antibody may be suitable for sandwich ELISA applications after pairing with a suitable detection antibody; validate in the relevant sample system.
• If using mouse samples, verify cross-reactivity due to the known human/mouse reactivity of this polyclonal antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated raw materials for IVD assay development. With this ACAP2 polyclonal antibody, researchers can accelerate studies of membrane trafficking and GTPase signaling. Our one-stop supply network offers antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare-target sourcing—helping you move from concept to clinic efficiently.