Anti-ACADS / SCAD Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P16219

Core Product Specifications and Parameters

Biological Background: ACADS/SCAD Function and Localization

• Also known as butyryl-CoA dehydrogenase, it is a mitochondrial enzyme belonging to the acyl-CoA dehydrogenase family.
• Catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway, converting straight-chain fatty acyl-CoA thioesters into trans-2-enoyl-CoA.
• Shows substrate specificity for saturated fatty acyl-CoA esters with chain lengths of 4 to 6 carbons. Related references: PMID:11134486 PMID:21237683
• This enzymatic reaction is FAD-dependent, serving as an electron acceptor in the dehydrogenation process.
• Localizes to the mitochondrial matrix, consistent with its role in fatty acid catabolism.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity.
• Mutations in ACADS are associated with short-chain acyl-CoA dehydrogenase deficiency, an inborn error of metabolism.
• Contains a transit peptide that directs its import into mitochondria.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide corresponding to amino acids 313-412 of human ACADS/SCAD (P16219). This region may determine epitope specificity.
• For Western blot, the expected molecular weight is approximately 44 kDa. Consider using appropriate reducing conditions and molecular weight markers.
• In immunohistochemistry (IHC-P), proper antigen retrieval and detection methods should be optimized for the target tissue.
• Cross-reactivity is reported with human, mouse, and rat. Validate the antibody for your specific species and sample type.
• For ELISA, employ the appropriate secondary detection system and include controls for accurate quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined source for validated IVD raw materials, including monoclonal antibodies like this anti-ACADS/SCAD antibody. Our portfolio spans antibody pairs, bulk ancillary reagents, and tailored support for rare targets, facilitating your development from bench to clinic. Rely on our expertise in molecular biology and B2B sourcing to accelerate your assay and diagnostic projects.