Anti-ACADS Rabbit Polyclonal Antibody for WB, IP, ELISA - P16219

Core Product Specifications and Parameters

Biological Background: ACADS Function and Localization

• ACADS encodes short-chain specific acyl-CoA dehydrogenase (SCAD), a mitochondrial matrix enzyme also known as butyryl-CoA dehydrogenase.
• SCAD catalyzes the first step of mitochondrial fatty acid beta-oxidation, specifically acting on straight-chain acyl-CoAs with saturated 4 to 6 carbon long primary chains, removing hydrogen to form trans-2-enoyl-CoA and enabling energy production from fats. Related references: PMID:11134486, PMID:21237683
• It belongs to the acyl-CoA dehydrogenase family of flavoproteins, utilizing FAD as a cofactor for its oxidoreductase activity.
• SCAD is nuclear-encoded and imported into the mitochondrial matrix via a transit peptide, as indicated by proteomics and sequence analysis.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or stability.
• Variants in ACADS are associated with short-chain acyl-CoA dehydrogenase deficiency, a metabolic disorder, underscoring its clinical relevance in mitochondrial fatty acid metabolism.
• The protein is part of the lipid metabolism and fatty acid metabolism pathways, as reflected in its UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1–260) of human ACADS; consider that reactivity may be influenced by post-translational modifications or partial proteolysis in the mitochondrial matrix.
• For western blotting, a band near 44 kDa is expected; always run appropriate positive (e.g., human, mouse, rat tissue lysates) and negative controls to confirm specificity.
• When performing immunoprecipitation or ELISA, optimization of antibody concentration and incubation conditions is recommended, as polyclonal antibodies can exhibit lot-to-lot variability.
• Enrichment of mitochondrial fractions may enhance signal due to the protein's subcellular localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated polyclonal antibodies like this anti-ACADS rabbit antibody for fatty acid oxidation studies. We offer one-stop sourcing from antibody pairs to bulk ancillary reagents, streamlining your development from concept to clinic with a focus on rare-targets and metabolic research tools.