Anti-ACADS Polyclonal Antibody for WB, IHC-P, ELISA - P16219

Core Product Specifications and Parameters

Biological Background: ACADS Function and Localization

• ACADS (also known as SCAD or butyryl-CoA dehydrogenase) is a mitochondrial enzyme belonging to the acyl-CoA dehydrogenase family. It catalyzes the first step of the beta-oxidation cycle, specifically the dehydrogenation of short-chain fatty acyl-CoA esters with saturated chains of 4 to 6 carbons. Related references: PMID:11134486 PMID:21237683
• The enzyme is localized in the mitochondrial matrix, where it participates in the breakdown of fatty acids to produce acetyl-CoA and energy.
• ACADS functions as a flavoprotein, requiring FAD as a cofactor for its oxidoreductase activity.
• The protein undergoes post-translational modifications such as acetylation and phosphorylation, which may modulate its activity and stability.
• Mutations in the ACADS gene cause short-chain acyl-CoA dehydrogenase deficiency, a metabolic disorder characterized by impaired fatty acid oxidation.
• The protein is synthesized as a precursor with an N-terminal mitochondrial transit peptide, which is cleaved to yield the mature enzyme.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-260), which includes the mitochondrial transit peptide; consider that the mature protein may show a slightly lower molecular weight than the precursor in WB.
• For WB, a band around 44 kDa is expected; optimize sample preparation and gel percentage to resolve the precursor if needed.
• For IHC-P, antigen retrieval may be required, and validate staining patterns with known positive controls.
• For ELISA, use recombinant ACADS protein or tissue lysates as a positive control to establish assay conditions.
• Verify antibody reactivity in your specific samples, as cross-reactivity with mouse and rat is indicated but may differ in applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for fatty acid metabolism research. Our sourcing expertise extends to rare targets, ensuring seamless procurement from concept to clinic. Partner with CamelBio to strengthen your supply chain with this anti-ACADS polyclonal antibody and related raw materials.