Anti-ACADM (MCAD) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P11310

Core Product Specifications and Parameters

Biological Background: ACADM Function and Localization

• Medium-chain specific acyl-CoA dehydrogenase (MCAD, encoded by ACADM) is a mitochondrial matrix enzyme that catalyzes the rate-limiting first step of fatty acid β-oxidation: the FAD-dependent dehydrogenation of fatty acyl-CoA thioesters to trans-2-enoyl-CoA, with electrons transferred to the electron transfer flavoprotein (ETF). ETF subsequently passes electrons to the mitochondrial respiratory chain. Related references: PMID:1970566, PMID:21237683, PMID:2251268, PMID:15159392, PMID:25416781

• MCAD displays substrate preference for medium-chain saturated fatty acyl-CoAs (C6–C12), but can also act on longer chains (C14, C16). Related references: PMID:1970566, PMID:21237683, PMID:2251268

• Localized to the mitochondrial matrix, ACADM is ubiquitously expressed with highest levels in heart, liver, and skeletal muscle.

• Mutations in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCADD), a common inborn error of fatty acid metabolism.

• The protein contains a FAD cofactor and undergoes post-translational modifications including acetylation and phosphorylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant protein covering residues 1-220 of human ACADM, suggesting the antibody may recognize multiple N-terminal epitopes. For western blot, expect a band at approximately 47 kDa; validate detection using mitochondria-rich samples such as liver or muscle lysates.

• This antibody has been tested in WB, IF/ICC, and ELISA. For IF/ICC, mitochondrial staining patterns should be confirmed with organelle markers. Optimize dilutions empirically for each application and cell/tissue type.

• Cross-reactivity with mouse and rat is indicated, making the antibody suitable for translational studies; however, individual verification in these species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material sourcing with a broad portfolio of research and diagnostic antibodies, including this anti-ACADM polyclonal antibody validated for multiple applications and species. We support fatty acid oxidation and metabolic disorder research by supplying validated antibody pairs, bulk ancillary reagents, and customized raw material procurement for rare or emerging targets. Contact us to discuss your diagnostic development needs—from target selection to clinical validation.