Anti-ACADL Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P28330

Core Product Specifications and Parameters

Biological Background: ACADL Function and Localization

• ACADL (acyl-CoA dehydrogenase long chain) is also known as LCAD and ACAD4, a mitochondrial enzyme essential for fatty acid beta-oxidation.
• It catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway, performing the alpha,beta-dehydrogenation of long-chain fatty acyl-CoA thioesters to trans-2-enoyl-CoA using electron transfer flavoprotein (ETF) as the electron acceptor. Related references: PMID:17564966 PMID:21237683 PMID:24591516
• Each cycle of beta-oxidation shortens the fatty acid chain by two carbons, producing acetyl-CoA, FADH₂, and NADH, which fuel the citric acid cycle and oxidative phosphorylation for ATP production. Related references: PMID:17564966 PMID:26474213
• ACADL prefers saturated and unsaturated fatty acyl-CoAs with chain lengths of 8–24 carbons, with highest activity towards 8–18 carbon substrates. Related references: PMID:17564966 PMID:21237683 PMID:32389575
• The enzyme also oxidizes unusual fatty acids such as docosahexaenoate (DHA) and branched-chain fatty acids, facilitated by its enlarged substrate-binding cavity. Related references: PMID:17564966 PMID:26474213 PMID:38839792
• ACADL is localized in the mitochondrial matrix.
• ACADL plays a primary role in lung alveolar type II cells, where it provides energy for surfactant production via fatty acid oxidation. Related references: PMID:17564966 PMID:24591516
• Besides lung, ACADL mRNA and protein are detected in prostate, thyroid, kidney, heart, and muscle.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 31-210, a region that includes part of the N-terminal domain; the antibody is expected to recognize the full-length ACADL protein.
• For Western blot (WB), the observed band may be around 48 kDa (consistent with the predicted molecular weight of 47.7 kDa), but post-translational modifications could cause slight shifts.
• IHC-P may require antigen retrieval; optimization is recommended depending on tissue type and fixation methods.
• Cross-reactivity with mouse and rat suggests utility in rodent models; however, validate in the relevant sample system before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents for mitochondrial and metabolic disease research. For ACADL-related projects, we provide polyclonal and monoclonal antibodies, antigen sources, and technical support to streamline your diagnostic development. Contact us to explore custom procurement solutions for your rare target needs.