Anti-ACAD8 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9UKU7

Core Product Specifications and Parameters

Biological Background: ACAD8 Function and Localization

• ACAD8 (Isobutyryl-CoA dehydrogenase, mitochondrial) is also known as Activator-recruited cofactor 42 kDa component (ARC42) and Acyl-CoA dehydrogenase family member 8 (ACAD-8).
• It catalyzes the conversion of 2-methylpropanoyl-CoA (isobutyryl-CoA) to (2E)-2-methylpropenoyl-CoA, a critical step in the mitochondrial valine catabolic pathway. Related references: PMID:11013134, PMID:12359132, PMID:16857760
• To a lesser extent, ACAD8 also oxidizes (2S)-2-methylbutanoyl-CoA, displaying broader substrate specificity within branched-chain amino acid metabolism. Related references: PMID:11013134, PMID:12359132
• ACAD8 belongs to the acyl-CoA dehydrogenase family, a group of flavoproteins involved in fatty acid and amino acid oxidation.
• The protein localizes exclusively to the mitochondrion, consistent with its role in energy metabolism.
• ACAD8 is broadly expressed in heart, lung, brain, skeletal muscle, pancreas, and placenta, with weaker expression in liver and kidney.
• Post-translational modifications include acetylation, and alternative splicing gives rise to multiple isoforms. It is associated with disease variants (isobutyryl-CoA dehydrogenase deficiency) and is classified under branched-chain amino acid catabolism.

Experimental Guidance and Technical Tips

• The immunogen spans residues 24–415 of human ACAD8, covering the mature protein after mitochondrial transit peptide cleavage; consider this for epitope accessibility in native vs. denaturing assays.
• For Western blot, a band around 45 kDa is expected; validate using relevant tissue lysates (e.g., heart, skeletal muscle) from human, mouse, or rat.
• IHC-P may require antigen retrieval optimization; positive controls should include tissues with high mitochondrial content or known ACAD8 expression.
• ELISA performance depends on the specific plate format and detection system; pairing with appropriate secondary antibodies is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for IVD development. This ACAD8 antibody is suited for metabolic disease research and assay design; we assist with rare‑target raw‑material sourcing and offer technical consultation to help bridge your project from concept to clinic.