Anti-ACAD8 Polyclonal Antibody for WB, ELISA - Q9Z0M6

Core Product Specifications and Parameters

Biological Background: ACAD8 Function and Localization

• Adhesion G protein-coupled receptor E5 (CD97) functions as a receptor involved in both adhesion and signaling processes early after leukocyte activation.
• It plays an essential role in leukocyte migration, mediating cell-cell interactions and immune cell trafficking.
• Belonging to the adhesion GPCR family, it features a long extracellular domain with multiple EGF-like repeats that facilitate cell adhesion.
• Alternative splicing generates several isoforms with variations in the number of EGF-like domains and subunit composition, potentially modulating ligand binding and downstream signaling.
• The protein localizes primarily to the cell membrane as a multi-pass membrane protein; however, a secreted form can be released into the extracellular space via proteolytic cleavage.
• Expression is predominantly found in immune cells, with high levels detected in the lung, thymus gland, spleen, and bone marrow, particularly on myeloid cells and subsets of T-, B-, and NK cells.
• Post-translational modifications include glycosylation, phosphorylation, and formation of disulfide bonds, which are critical for structural stability and receptor function.
• Key associated keywords: Alternative splicing, Calcium, Cell adhesion, Cell membrane, Disulfide bond, EGF-like domain, G protein-coupled receptor, Glycoprotein, Membrane, Phosphoprotein, Receptor, Repeat, Secreted, Signal, Transducer, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 24-415 of human ACAD8; consider sequence homology alignment across human, mouse, and rat to anticipate cross-reactivity.
• For Western blotting, prepare whole cell lysates from leukocyte-rich tissues (e.g., spleen, thymus) or cultured immune cells, and use a suitable reducing agent to account for disulfide bonds in the extracellular domain.
• In ELISA, use the recombinant immunogen as a positive control and optimize coating conditions to ensure proper antigen presentation; perform cross-reactivity tests with mouse and rat proteins to validate detection.
• Given the target's predicted molecular weight of 45 kDa (full-length ACAD8 isoform), monitor for potential cleavage products or isoforms, especially in secreted fractions.
• Validate the antibody in the relevant biological context, such as leukocyte adhesion assays or migration studies, to confirm functional application reliability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and researchers with streamlined access to a broad portfolio, including this anti-ACAD8 polyclonal antibody for leukocyte adhesion and migration studies. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, supporting your project from concept to clinical application.