Anti-ACAA2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P42765

Core Product Specifications and Parameters

Biological Background: ACAA2 Function and Localization

• ACAA2 (acetyl-CoA acyltransferase 2), also known as 3-ketoacyl-CoA thiolase, mitochondrial acetyl-CoA acetyltransferase, or beta-ketothiolase, is a key enzyme encoded by the ACAA2 gene in humans.
• It catalyzes the final step of mitochondrial fatty acid beta-oxidation, cleaving medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and shortened acyl-CoA, thereby playing a central role in energy production from fats.
• ACAA2 can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA, suggesting involvement in ketone body biosynthesis.
• The protein localizes to the mitochondrion, consistent with its function in lipid metabolism and energy homeostasis.
• ACAA2 has been shown to abolish BNIP3-mediated apoptosis and mitochondrial damage, indicating a potential role in cell survival pathways. Related references: PMID:18371312
• In addition to its thiolase activity, ACAA2 displays hydrolase activity on various fatty acyl-CoAs, which may contribute to acetate production as a side reaction of beta-oxidation. Related references: PMID:25478839
• Post-translational modifications include acetylation and phosphorylation, which may regulate its enzymatic activity and subcellular dynamics.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-250 of human ACAA2. Consider this when designing validation assays or epitope mapping studies.
• For Western blot (WB) applications, a band at approximately 42 kDa is expected; validate the band pattern in your cell or tissue lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval conditions and antibody dilution to achieve specific mitochondrial staining.
• When using ELISA, ensure that the coating antigen and detection system are compatible with the host species (rabbit) and target recognition.
• Cross-reactivity with mouse and rat has been reported; verify reactivity in the relevant species and sample type for your study.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents, to accelerate your development of diagnostic assays and research tools. With this anti-ACAA2 antibody, we support projects focused on mitochondrial metabolism, fatty acid oxidation disorders, and related areas. As a trusted partner, CamelBio can also source rare targets and provide bulk supply solutions to meet your program's needs from concept to clinic.