Anti-ACAA2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P42765

Core Product Specifications and Parameters

Biological Background: ACAA2 Function and Localization

• ACAA2, also known as 3-ketoacyl-CoA thiolase or acetyl-CoA acetyltransferase, is a mitochondrial enzyme encoded by the ACAA2 gene.
• It catalyzes the final step of mitochondrial fatty acid β-oxidation, thiolytically cleaving medium- to long-chain 3-oxoacyl-CoAs to produce acetyl-CoA and a shortened fatty acyl-CoA.
• ACAA2 can also condense two acetyl-CoA molecules to form acetoacetyl-CoA, potentially contributing to ketone body synthesis.
• The enzyme exhibits hydrolase activity on various fatty acyl-CoAs, generating acetate in a side reaction to β-oxidation. Related references: PMID:25478839
• ACAA2 has been shown to abolish BNIP3-mediated apoptosis and mitochondrial damage. Related references: PMID:18371312
• Subcellularly, ACAA2 localizes to the mitochondrion, consistent with its role in fatty acid metabolism.
• It belongs to the thiolase family of acyltransferases and is subject to acetylation and phosphorylation, as indicated by proteomics identification and the presence of a 3D-structure in UniProt.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 17-271 of human ACAA2, which may contribute to cross-reactivity with mouse and rat orthologs; consider sequence alignment if reactivity with other species is required.
• For Western blotting, a band around 42 kDa is expected; ensure the use of appropriate molecular weight markers and validate specificity in the relevant tissue or cell line.
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized, and the antibody should be validated in appropriate positive and negative control tissues.
• For ELISA, the antibody may be used in capture or detection formats; optimization of coating concentration and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents for diagnostic development. This anti-ACAA2 antibody supports fatty acid metabolism and mitochondrial research, and CamelBio can assist with bulk supply, custom conjugation, and rare-target sourcing to streamline your assay development.