Anti-ACAA2 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P42765

Core Product Specifications and Parameters

Biological Background: ACAA2 Function and Localization

• Encodes mitochondrial 3-ketoacyl-CoA thiolase (also known as acetyl-CoA acetyltransferase, beta-ketothiolase, T1), a key enzyme in fatty acid beta-oxidation.
• Catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a shortened acyl-CoA, the final step of mitochondrial beta-oxidation.
• Exhibits hydrolase activity on various fatty acyl-CoAs, potentially generating acetate as a side reaction. Related references: PMID:25478839
• Also catalyzes the condensation of two acetyl-CoA molecules to acetoacetyl-CoA, suggesting involvement in ketone body metabolism.
• Abolishes BNIP3-mediated apoptosis and mitochondrial damage, indicating a role in cell survival. Related references: PMID:18371312
• Localizes to the mitochondrion.
• Post-translationally modified by acetylation and phosphorylation; contains an N-terminal transit peptide for mitochondrial import.
• Belongs to the thiolase family and is essential for energy production from fatty acids.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 298–397 near the C-terminus; verify epitope accessibility in your sample system.
• For Western blot, expect a specific band at approximately 42 kDa; use mitochondrial-enriched lysates to enhance signal.
• In immunofluorescence/ICC, the antibody should detect mitochondrial localization; consider co-staining with a mitochondrial marker.
• ELISA performance may be optimized by using the immunizing peptide as a positive control; validate dilution factors empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. For fatty acid metabolism and mitochondrial research areas, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our expert team can also assist in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.