Anti-ACAA1 Rabbit Polyclonal Antibody for WB, ELISA - P09110

Core Product Specifications and Parameters

Biological Background: ACAA1 Function and Localization

• Encodes peroxisomal 3-ketoacyl-CoA thiolase (also known as beta-ketothiolase), a key enzyme in fatty acid beta-oxidation that catalyzes the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs). Related references: PMID:11734571 PMID:2882519
• Substrates include short, medium, long, and very long chain 3-oxoacyl-CoAs, highlighting its broad substrate spectrum essential for energy metabolism. Related references: PMID:11734571 PMID:2882519
• May participate in polyunsaturated fatty acid (PUFA) biosynthesis, specifically in the peroxisomal beta-oxidation step that converts tetracosahexaenoic acid (THA) to docosahexaenoic acid (DHA), a critical omega-3 fatty acid. Related references: PMID:11734571 PMID:2882519
• Localizes exclusively to the peroxisome, where it carries out its catalytic function; the N-terminal region contains a peroxisomal targeting signal (transit peptide) that directs import into the organelle.
• Subject to alternative splicing and phosphorylation, as indicated by UniProt keywords; these post-translational modifications may regulate enzyme activity, localization, or stability.
• Classified as an acyltransferase and a transferase, reflecting its mechanism of transferring an acyl group during the thiolysis reaction.
• Structurally, the full-length protein has a molecular weight of approximately 44 kDa (calculated 44,292 Da), consistent with the 44 kDa band observed in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 27–300, covering the catalytic core and substrate-binding domains; consider epitope availability in different sample preparations.
• For Western blotting, a specific band near 44 kDa is expected; validate in the relevant sample system (e.g., human or rat peroxisome-enriched fractions).
• Cross-reactivity with rat suggests conserved epitopes; however, always verify species-specific detection in your assay conditions.
• This polyclonal antibody may recognize multiple isoforms due to alternative splicing; if needed, confirm isoform specificity using recombinant controls.
• In ELISA applications, coat with recombinant ACAA1 protein and follow standard indirect detection protocols; optimize antibody dilution empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, CROs, and research labs with reliable access to IVD raw materials and technical support. This anti-ACAA1 polyclonal antibody, targeting a central enzyme in peroxisomal fatty acid oxidation, is backed by our supply capability for validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare target sourcing. Our integrated platform helps streamline your assay development from concept to clinic.