Anti-ACAA1 Polyclonal Antibody for WB, ELISA - P09110

Core Product Specifications and Parameters

Biological Background: ACAA1 (3-ketoacyl-CoA thiolase, peroxisomal) Function and Localization

• ACAA1 encodes peroxisomal 3-ketoacyl-CoA thiolase, a key enzyme also referred to as acetyl-CoA acyltransferase, beta-ketothiolase, or peroxisomal 3-oxoacyl-CoA thiolase.
• This enzyme catalyzes the thiolytic cleavage of straight-chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs) and is essential for peroxisomal beta-oxidation of fatty acids.
  Related references:
  PMID:11734571
  PMID:2882519
• It processes short, medium, long, and very long chain 3-oxoacyl-CoAs, highlighting its broad substrate specificity (PubMed:11734571, PubMed:2882519).
  Related references:
  PMID:11734571
  PMID:2882519
• ACAA1 may also contribute to polyunsaturated fatty acid (PUFA) biosynthesis and regulate docosahexaenoic acid (DHA) levels by beta-oxidizing tetracosahexaenoic acid (THA) in peroxisomes.
• The protein localizes to the peroxisome, directed by an N-terminal transit peptide.
• Alternative splicing yields multiple isoforms, and the enzyme is subject to phosphorylation; its three-dimensional structure has been experimentally determined.
• Belongs to the acyltransferase family and is classified under fatty acid metabolism and lipid metabolism pathways.

Experimental Guidance and Technical Tips

• The immunogen sequence covers amino acids 210-310 of human ACAA1; consider its conservation when assessing reactivity across species (e.g., reported mouse cross-reactivity).
• For Western blotting, a band around 44 kDa is expected; validate antibody specificity using appropriate positive and negative controls.
• In ELISA, optimize coating antigen and antibody dilution for the specific assay format.
• Since ACAA1 is a peroxisomal enzyme, sample preparation may require enrichment of peroxisomal fractions for optimal detection.
• As a rabbit polyclonal antibody, consider potential lot-to-lot variability and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including polyclonal antibodies like this ACAA1 antibody for peroxisomal fatty acid metabolism studies. We offer validated antibody pairs, optimized recombinant proteins, and ancillary reagents to streamline assay development. For custom or bulk requirements, our team can source rare targets and deliver tailored solutions to meet your project timelines.