Anti-ABR Rabbit Polyclonal Antibody for WB and ELISA - Q12979

Core Product Specifications and Parameters

Biological Background: ABR Function and Localization

• ABR, also known as Active breakpoint cluster region-related protein or MDB, is a member of the breakpoint cluster region (BCR) family of proteins. It is a multidomain GTPase regulator.
• Functions as a GTPase-activating protein (GAP) for RAC1, RAC2, and CDC42, accelerating GTP hydrolysis and down-regulating their active forms. Related references: PMID:17116687 PMID:7479768
• Also possesses Dbl homology (DH) domain with guanine nucleotide exchange factor (GEF) activity, promoting conversion of CDC42, RHOA, and RAC1 from GDP- to GTP-bound state. Related references: PMID:7479768
• Acts as a negative regulator of neuronal RAC1 activity and modulates macrophage functions such as CSF-1 directed motility and phagocytosis (By similarity).
• Subcellular localization: found at dendritic spines, axons, and synapses.
• Expression is highly enriched in the brain, with weaker expression in heart, lung, and muscle.
• Classified as a phosphoprotein and expressed as multiple isoforms due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to the C-terminal region; thus, the antibody may recognize ABR isoforms containing this sequence. Validate in the relevant sample system.
• For Western blot, a band around 98 kDa is expected. Consider using brain tissue lysate as a positive control due to high ABR expression.
• The antibody cross-reacts with mouse and rat; it may be suitable for studies in rodent models. Confirm specificity in the species of interest before use.
• For ELISA, optimize coating and detection conditions empirically to achieve optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to high-quality IVD raw materials. This anti-ABR polyclonal antibody supports research into GTPase signaling, neuronal function, and macrophage biology. Our comprehensive supply portfolio includes validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents, helping you streamline assay development from concept to clinic.