Anti-ABLIM3 Polyclonal Antibody for WB and ELISA - O94929

Core Product Specifications and Parameters

Biological Background: ABLIM3 Function and Localization

• ABLIM3 (Actin-binding LIM protein 3) is a scaffold protein encoded by the ABLIM3 gene (Gene ID: 22885) in humans.
• It may act as a scaffold protein and stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.
• Predominantly expressed in heart and brain, suggesting roles in cardiac and neural development or function.
• Located in the cytoplasm.
• Contains LIM domains, which are involved in protein-protein interactions and bind zinc (metal-binding).
• Subject to post-translational modifications including acetylation, methylation, and phosphorylation, as identified by proteomics.
• Alternative splicing generates multiple isoforms.
• The protein has a molecular weight of approximately 78 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350-520 of human ABLIM3; consider validating specificity in the relevant sample system.
• For Western blot, the predicted molecular weight is 78 kDa; include appropriate positive controls and molecular weight markers.
• ELISA application may be used to detect ABLIM3; optimize antibody concentration and assay conditions.
• The antibody cross-reacts with mouse and rat ABLIM3, indicating conserved epitopes; useful for comparative animal model studies.
• ABLIM3 is highly expressed in heart and brain; these tissues can serve as positive controls.
• As a rabbit polyclonal antibody, lot-to-lot variability may occur; re-validate performance for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop partner for IVD raw materials, supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This anti-ABLIM3 antibody supports cardiac and neuroscience research applications. From concept to clinic, CamelBio provides reliable sourcing solutions tailored for diagnostic manufacturers and laboratories.