Anti-ABLIM2 Rabbit Polyclonal Antibody for WB, ELISA - Q6H8Q1

Core Product Specifications and Parameters

Biological Background: ABLIM2 Function and Localization

• ABLIM2 (Actin-binding LIM protein 2) is a member of the actin-binding LIM protein family, characterized by LIM and zinc-binding domains that mediate protein-protein interactions.
• The protein functions as a scaffold that may stimulate ABRA (actin-binding Rho-activating protein) activity and ABRA-dependent SRF (serum response factor) transcriptional activity.
• ABLIM2 is predominantly localized in the cytoplasm, consistent with its role in cytoskeletal organization and actin dynamics.
• It is highly expressed in skeletal muscle, suggesting a specialized role in muscle cell structure or function.
• Multiple post-translational modifications have been identified, including phosphorylation, which may regulate its activity.
• The protein contains zinc-binding LIM domains that are essential for its function; zinc binding and metal coordination are key features.
• An alternative splicing isoform exists, potentially generating variant functions or tissue-specific expression patterns.

Experimental Guidance and Technical Tips

• The immunogen spans a central region (aa 74–309) of human ABLIM2, raising polyclonal antibodies that recognize multiple epitopes; this may provide robust detection across isoforms and species.
• For Western blot, use tissue or cell lysates rich in ABLIM2 (e.g., skeletal muscle); consider validating the antibody at a dilution that yields a clear band near 68 kDa.
• Cross-reactivity with mouse and rat has been reported, enabling translational studies; however, always verify specificity in the relevant sample system.
• In ELISA, the polyclonal nature may enhance sensitivity for antigen capture; optimize coating and detection conditions for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable sourcing for IVD raw materials, including validated polyclonal and monoclonal antibodies like anti-ABLIM2 for muscle biology and cytoskeletal studies. From bulk ancillary reagents to rare target antibody development, CamelBio supports your project from concept to clinic with comprehensive technical guidance. As a one-stop partner, we streamline procurement for all your IVD raw material needs.