Anti-ABI4 Rabbit Polyclonal Antibody for WB and ELISA - A0MES8

Core Product Specifications and Parameters

Biological Background: ABI4 Function and Localization

• ABI4 is an ethylene-responsive transcription factor (also known as ABA INSENSITIVE 4, GIN6, ISI3, SAN5, SIS5, SUN6) that regulates gene expression in response to stress and developmental cues.
• It binds to GCC-box pathogenesis-related promoter elements and S-box (CACTTCCA) elements of photosynthesis-associated nuclear genes, acting as a transcriptional repressor.
• ABI4 is a key component of the abscisic acid (ABA) signaling pathway, regulating seed germination, lateral root inhibition, osmotic stress responses, and ABA sensitivity.
• It also participates in sugar signaling, including hexokinase-dependent glucose responses, sucrose sensing, and trehalose-mediated root inhibition, and acts as a negative regulator of low water potential-induced proline accumulation.
• The protein is involved in plant defense, contributing to beta-amino-butyric acid (BABA)-primed resistance against necrotrophic pathogens (e.g., Alternaria brassicicola and Plectosphaerella cucumerina) and resistance to Sclerotinia sclerotiorum via ABA-mediated stomatal closure.
• Subcellular localization is nuclear, consistent with its role as a transcription factor.
• Tissue-specific expression is observed in seeds (embryo), seedlings (vascular tissues, hypocotyl, cotyledons, root cap, and quiescent center), indicating roles in early development and stress adaptation.
• Keywords associated with ABI4 include: Abscisic acid signaling pathway, DNA-binding, Ethylene signaling pathway, Nucleus, Plant defense, Repressor, Stress response, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of Arabidopsis ABI4. Ensure that your experimental system expresses this region; use sequence alignment if testing cross-reactivity in other species.
• For Western blot (WB) analysis, the predicted molecular weight is 36 kDa. Validate the band in tissues or treatments known to express ABI4 (e.g., seedlings, ABA-treated samples).
• For ELISA, consider using recombinant ABI4 protein or the immunizing peptide as a positive control to establish assay conditions.
• Cross-reactivity has been verified with Arabidopsis thaliana. For other plant species, check sequence homology and perform a pilot experiment.
• Given ABI4’s role in stress and hormone signaling, consider inducing expression with ABA, salt, or osmotic stress to enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. This anti-ABI4 antibody, targeting a central regulator in plant hormone and stress signaling, is part of our broad portfolio of high-quality antibodies and ancillary reagents for plant biology and agrochemical research. We also offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk reagents, and rare target sourcing to streamline your development pipeline.