Anti-ABHD5 Monoclonal Antibody for WB, ELISA - Q8WTS1

Core Product Specifications and Parameters

Biological Background: ABHD5 Function and Localization

• ABHD5, officially designated 1‑acylglycerol‑3‑phosphate O‑acyltransferase ABHD5 and also known as CGI‑58, is a 39 kDa lipid droplet‑binding protein and member of the α/β hydrolase superfamily.
• It functions as a coenzyme A‑dependent lysophosphatidic acid acyltransferase that preferentially utilizes 1‑oleoyl‑lysophosphatidic acid and arachidonoyl‑CoA to produce phosphatidic acid, a central intermediate in glycerolipid biosynthesis. Related references: PMID:18606822
• ABHD5 co‑activates the triglyceride lipase PNPLA2 (ATGL), thereby promoting triacylglycerol hydrolysis and controlling lipid droplet size and fusion. Related references: PMID:16679289
• The protein is essential for keratinocyte differentiation, linking epidermal lipid metabolism to skin barrier integrity. Related references: PMID:18832586
• Subcellularly, ABHD5 partitions between the cytosol and the surface of lipid droplets, enabling direct contact with its lipase effector.
• It is widely expressed across human tissues, with prominent protein levels in liver, skeletal muscle, brain, skin, and lymphocytes.
• Disease associations and post‑translational modifications include acetylation, phosphorylation, and causal variants in neutral lipid storage disease with ichthyosis (Chanarin‑Dorfman syndrome) as well as neurosensory defects such as cataract and deafness.

Experimental Guidance and Technical Tips

• For Western blot, prepare lysates from human or rat cell lines or tissues known to express ABHD5, such as liver, skin fibroblasts, or keratinocytes. A specific band at approximately 39 kDa should be detected. Optimize blocking and antibody dilution to minimize background.
• Given the synthetic peptide immunogen (amino acids 100–200), the antibody likely recognizes a linear epitope; denaturing and reducing conditions are compatible.
• For ELISA, the coating antigen may be the immunizing peptide or recombinant ABHD5 protein; validate assay conditions with appropriate blank and negative controls.
• Cross‑reactivity with rat enables translational studies, but performance should be confirmed in the intended sample matrix.
• Because ABHD5 partitions between cytosol and lipid droplets, subcellular fractionation protocols may enhance detection in certain sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, offering reliable antibodies like this anti‑ABHD5 clone for lipid metabolism and dermatology research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal reagents, and ancillary raw materials to streamline your diagnostic development from concept to clinic.