Anti-ABHD4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8TB40

Core Product Specifications and Parameters

Biological Background: ABHD4 Function and Localization

• ABHD4 is an alpha/beta hydrolase domain-containing protein, also known as abhydrolase domain-containing protein 4 (ABH4).
• It functions as a lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE), contributing to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide, by generating glycerophospho-N-acyl ethanolamine (GP-NAE).
• It hydrolyzes substrates bearing saturated, monounsaturated, and polyunsaturated N-acyl chains.
• Shows no significant activity towards other lysophospholipids, such as lysophosphatidylcholine, lysophosphatidylethanolamine, and lysophosphatidylserine.
• Alternatively spliced transcript variants have been described.
• Proteomics studies have confirmed its expression.
• Belongs to the alpha/beta hydrolase superfamily and is involved in lipid degradation and metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 223-342 of human ABHD4; this region is conserved in mouse and rat, supporting cross-reactivity.
• For Western blot, expect a band at approximately 39 kDa; consider using positive control lysates from human, mouse, or rat tissues/cells.
• For IF/ICC, validate in human cell lines; optimization may be needed for mouse and rat samples due to potential cross-reactivity differences.
• The antibody is suitable for ELISA; empirical determination of coating and detection conditions is recommended.
• As with any polyclonal antibody, lot-to-lot variability can occur; include appropriate controls and validate performance in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories one-stop access to high-quality IVD raw materials, including validated antibodies like this anti-ABHD4 polyclonal antibody. Whether you require bulk ancillary reagents or rare target sourcing for lipid metabolism and endocannabinoid signaling studies, we support your entire project lifecycle—from concept to clinical application.