Anti-ABCG8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H221

Core Product Specifications and Parameters

Biological Background: ABCG8 Function and Localization

• ABCG8 (Sterolin-2) is a member of the ATP-binding cassette (ABC) transporter superfamily, sub-family G, and functions exclusively as an obligate heterodimer with ABCG5 to mediate sterol transport.
• The ABCG5/ABCG8 heterodimer facilitates Mg²⁺- and ATP-dependent transport of cholesterol and plant sterols across the apical membrane of enterocytes and hepatocytes, playing a critical role in dietary cholesterol absorption and biliary sterol excretion. Related references: PMID:11099417 PMID:11452359 PMID:15054092
• The complex exhibits intrinsic ATPase activity that is essential for its transport function. Related references: PMID:16893193 PMID:20210363 PMID:27144356
• ABCG8 is predominantly expressed in the liver, with low levels in the small intestine and colon, and very low levels in brain, heart, and spleen.
• Subcellular localization is at the apical cell membrane, consistent with its role in vectorial sterol transport.
• Keywords associated with ABCG8 include alternative splicing, glycoprotein, transmembrane, disease variant (mutations linked to sitosterolemia), and lipid transport.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 320 amino acids of human ABCG8; use this information when interpreting band sizes in WB, which may appear near the predicted 76 kDa.
• For WB, consider using positive controls such as liver or intestinal cell lysates, where ABCG8 expression is expected; validate in your specific sample system.
• In IF/ICC, anticipate apical membrane staining in polarized cells (e.g., hepatocyte-derived lines like HepG2 or intestinal Caco-2 cells); co-staining with an apical marker may aid localization.
• Since ABCG8 functions as an obligate heterodimer with ABCG5, functional assays may require co-expression of both proteins; for overexpression studies, consider co-transfection to recapitulate the native complex.
• For ELISA, the antibody may be suitable for capture or detection of recombinant ABCG8; optimal dilutions and matrix compatibility should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined sourcing solution for high-quality IVD raw materials. This anti-ABCG8 polyclonal antibody supports research into cholesterol metabolism and sterol transport disorders. Beyond antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—helping you advance from concept to clinic.