Anti-ABCG2 Rabbit Monoclonal Antibody for WB, ELISA - Q9UNQ0

Core Product Specifications and Parameters

Biological Background: ABCG2 Function and Localization

• ABCG2, also known as breast cancer resistance protein (BCRP), is a member of the ATP-binding cassette (ABC) sub-family G. It encodes a broad substrate specificity ATP-dependent transporter with multiple alternatively spliced isoforms.
• ABCG2 functions as an efflux pump that actively extrudes a wide range of xenobiotics, physiological compounds, and dietary toxins, thereby conferring multidrug resistance by exporting chemotherapeutic agents such as mitoxantrone, methotrexate, and anthracyclines. Related references: PMID:11306452, PMID:12958161, PMID:12477054, PMID:15670731
• The transporter mediates porphyrin homeostasis by exporting protoporphyrin IX and heme, regulates sphingosine-1-phosphate signaling, serves as a urate exporter, and secretes sulfate-conjugated steroids and vitamins into multiple body fluids. Related references: PMID:20705604, PMID:23189181, PMID:20110355, PMID:19506252, PMID:20368174, PMID:22132962, PMID:12682043, PMID:28554189, PMID:30405239, PMID:39337371
• ABCG2 is predominantly localized at the apical cell membrane and is also detected on the mitochondrial membrane, consistent with its role in cellular efflux and organelle transport.
• The protein is highly expressed in the placenta, with lower levels found in small intestine, liver, colon, and brain, indicating a role in tissue barrier and excretion functions. Related references: PMID:9850061, PMID:9861027, PMID:12958161
• In addition to drug transport, ABCG2 may be involved in stem cell self-renewal and innate immunity by exporting itaconate from inflammatory macrophages, limiting lysosome biogenesis and antibacterial responses.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which are important for its stability and function as a transmembrane protein.

Experimental Guidance and Technical Tips

• The immunogen peptide (amino acids 100–200 of human ABCG2) is located within the intracellular domain; therefore, permeabilization may be necessary for detecting the epitope in fixed samples.
• In Western blot analysis, expect a main band near 72 kDa; however, post-translational modifications like glycosylation can cause slight migration shifts. Use membrane protein extraction protocols for optimal detection.
• When using ELISA, consider optimizing coating conditions, as the multispan transmembrane nature of ABCG2 may require specific buffers or detergents to expose the epitope.
• The antibody is validated for human reactivity; for cross-reactivity with other species, experimental verification is recommended.
• Due to ABCG2's expression in drug-resistant cancer cells and normal tissues like placenta, include appropriate positive and negative controls in your experiments.
• For functional studies, combine this antibody with inhibitors such as Ko143 to confirm ABCG2-specific activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for IVD assay development, including targets involved in drug resistance and ABC transporter research. As your dedicated one-stop sourcing partner, we streamline access to rare target raw materials and provide technical support from early-stage R&D to clinical application.