Anti-ABCG2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UNQ0

Core Product Specifications and Parameters

Biological Background: ABCG2 Function and Localization

• ABCG2 (ATP-binding cassette sub-family G member 2), also known as BCRP or CDw338, is a broad-specificity ATP-dependent transporter that extrudes physiological compounds, dietary toxins, and xenobiotics from cells.\nRelated references:\nPMID:11306452, PMID:12958161, PMID:19506252
• It plays a critical role in porphyrin homeostasis by exporting protoporphyrin IX from mitochondria to cytosol and from cytosol to extracellular space, and it also exports heme.\nRelated references:\nPMID:20705604, PMID:23189181
• ABCG2 is a multidrug resistance transporter that effluxes chemotherapeutic agents such as mitoxantrone, methotrexate, anthracyclines, and camptothecin, conferring resistance in cancer cells.\nRelated references:\nPMID:11306452, PMID:12477054, PMID:15670731
• The protein functions as a urate exporter in kidney and extrarenal tissues, contributing to urate homeostasis and implications in gout.\nRelated references:\nPMID:19506252, PMID:20368174, PMID:22132962
• It transports sulfate conjugates, including steroid sulfates like estrone 3-sulfate and DHEAS, thereby modulating hormone metabolism.\nRelated references:\nPMID:12682043, PMID:28554189, PMID:30405239
• ABCG2 is highly expressed in placenta, with low expression in liver, intestine, and brain, where it restricts xenobiotic penetration, including limiting fetal drug exposure and contributing to the blood-brain barrier.\nRelated references:\nPMID:9850061, PMID:9861027, PMID:12958161
• In inflammatory macrophages, ABCG2 exports itaconate, dampening antibacterial innate immune responses by limiting TFEB-dependent lysosome biogenesis.
• Subcellularly, ABCG2 localizes primarily to the apical cell membrane and mitochondrion membrane, consistent with its transport and detoxification functions.

Experimental Guidance and Technical Tips

• The immunogen peptide is located within amino acids 100–200, a cytoplasmic region; the antibody may detect both membrane and intracellular protein pools.
• For western blot, a band near 72 kDa is expected; use placental or drug-resistant cell lysates as positive controls and validate in your target tissue.
• In IF/ICC, permeabilize cells to allow access to the intracellular epitope; staining may appear at cell membranes and mitochondria.
• For ELISA, optimize antigen coating and antibody concentration; the polyclonal nature may increase sensitivity but requires careful titration.
• Cross-reactivity with mouse and rat has been reported; confirm species-specific detection and any background signals in your assay conditions.
• As with any polyclonal, lot-to-lot variability may occur; include appropriate controls and consider downstream assay-specific adjustments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a complete portfolio of IVD raw materials, from validated antibody pairs to optimized polyclonal antibodies and bulk ancillary reagents, supporting diagnostic manufacturers and research laboratories at every development stage. The ABCG2 polyclonal antibody enhances our transporter target collection, ideal for drug resistance studies, biomarker assays, and pharmacokinetic research. With integrated sourcing capabilities, CamelBio accelerates your path from concept to clinical application.