Anti-ABCG1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P45844

Core Product Specifications and Parameters

Biological Background: ABCG1 Function and Localization

• ABCG1 (ATP-binding cassette sub-family G member 1), also known as ABC8 and White protein homolog, is encoded by the ABCG1 gene and belongs to the ABC transporter superfamily.
• It functions as an ATP-dependent transporter that catalyzes the efflux of phospholipids (such as sphingomyelin), cholesterol, and its oxygenated derivatives like 7beta-hydroxycholesterol; this transport is ALB-dependent. Related references: PMID:17408620, PMID:24576892, PMID:16702602
• ABCG1 is an active component of the macrophage lipid export complex and prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol. Related references: PMID:17408620
• The protein may also participate in intracellular lipid transport processes and helps maintain cellular lipid homeostasis in various cell types, not limited to macrophages.
• Subcellularly, ABCG1 is localized to the endoplasmic reticulum membrane, Golgi apparatus membrane, and cell membrane.
• Post-translational modifications include palmitoylation, and the protein contains characteristic transmembrane helices and nucleotide-binding domains typical of ABC transporters.
• ABCG1 is expressed in several tissues with higher levels in macrophages; its expression is upregulated in macrophages from Tangier disease patients.
• Multiple alternatively spliced transcript variants have been reported for this gene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 6-120 of human ABCG1, targeting the N-terminal region. For Western blot, a band around 76 kDa is expected; validate in your sample system with appropriate loading controls.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), test antibody dilutions in known positive tissues or cells (e.g., macrophages or cell lines expressing ABCG1) and use appropriate fixation/permeabilization protocols.
• This polyclonal antibody is suitable for ELISA; consider pairing it with an appropriate detection antibody for sandwich ELISA applications.
• As a polyclonal reagent, lot-to-lot variability is possible; titration and inclusion of positive/negative controls are recommended for each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, technical services, and consulting—from concept to clinic. This anti-ABCG1 antibody fits into lipid transport and cardiovascular disease research areas, and we offer a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you need research tools or scalable production-grade reagents, CamelBio helps bridge your procurement needs efficiently.