Anti-ABCG1 Rabbit Monoclonal Antibody for WB, ELISA - P45844

Core Product Specifications and Parameters

Biological Background: ABCG1 Function and Localization

• ABCG1, also referred to as ATP-binding cassette transporter 8 or White protein homolog, is a member of the ATP-binding cassette (ABC) transporter G subfamily and functions as a half-transporter likely forming a homodimer.
• It catalyzes the ATP-dependent efflux of phospholipids such as sphingomyelin, cholesterol, and oxygenated cholesterol derivatives like 7beta-hydroxycholesterol (PubMed:17408620, PubMed:24576892). This lipid transport process is albumin-dependent (PubMed:16702602). Related references: PMID:17408620, PMID:24576892, PMID:16702602
• By exporting cytotoxic 7beta-hydroxycholesterol, ABCG1 prevents cell death, underscoring its role in maintaining cellular lipid homeostasis and protecting macrophages from oxysterol-induced apoptosis (PubMed:17408620). Related references: PMID:17408620
• ABCG1 localizes to the endoplasmic reticulum membrane, Golgi apparatus membrane, and cell membrane, enabling both intracellular lipid trafficking and plasma membrane lipid efflux.
• It is expressed in multiple tissues, with prominent expression in macrophages; notably, ABCG1 expression is upregulated in macrophages from patients with Tangier disease, linking it to cholesterol metabolism disorders.
• Post-translational palmitoylation of ABCG1, as annotated in UniProt, likely modulates its membrane association and functional activity.
• The ABCG1 gene undergoes alternative splicing, generating multiple protein isoforms that may have distinct regulatory or transport properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide derived from amino acids 100–200 of human ABCG1 (NP_004906.3), a region likely exposed in denatured samples, making the antibody potentially suitable for WB under reducing conditions.
• Given the predicted protein weight of 76 kDa, use an appropriate molecular weight marker range and optimize gel percentage (e.g., 8–10% SDS-PAGE) for optimal resolution of ABCG1 bands.
• Cross-reactivity with mouse and rat ABCG1 suggests epitope conservation; validate specificity using lysates from relevant cell types, such as macrophage lines or primary macrophages.
• For ELISA, consider using recombinant ABCG1 protein or the immunizing peptide as a coating antigen and include appropriate positive and negative controls.
• To confirm antibody specificity, incorporate ABCG1-overexpressing cell lysates or knockout/knockdown models and test a range of antibody dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, laboratories, and research institutes, offering one-stop access to IVD raw materials and technical support from concept to clinic. For studies in lipid metabolism and macrophage biology, we supply a full portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in navigating rare-target procurement such as ABCG1. Reach out to streamline your supply chain for discovery and diagnostic development.