Anti-ABCF2 Polyclonal Antibody for WB - Q9UG63

Core Product Specifications and Parameters

Biological Background: ABCF2 Function and Localization

• ABCF2 is an ATP-binding cassette sub-family F member, also known as Iron-inhibited ABC transporter 2, encoded by the ABCF2 gene.
• It features two nucleotide-binding domains (NBDs) with conserved ATP-binding motifs, classifying it as an ATPase.
• The protein is annotated with the keyword "Alternative splicing", indicating potential isoform diversity.
• Post-translational modifications include phosphorylation and acetylation, as highlighted by proteomics data.
• The calculated molecular weight is approximately 71 kDa, consistent with the experimentally observed band.
• The "Repeat" keyword suggests internal sequence repeats within the nucleotide-binding domains.

Experimental Guidance and Technical Tips

• For Western blot, a band at ~71 kDa is expected in human or mouse cell lysates. Consider using a positive control such as HeLa or NIH/3T3 cells.
• In immunofluorescence, fixation and permeabilization conditions should be optimized; the signal may localize to the cytoplasm or nucleus depending on cell type.
• For ELISA, coat with recombinant ABCF2 protein or peptide and include appropriate negative and positive controls to validate specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a one-stop sourcing solution for IVD raw materials. In the field of ABC transporter research, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. As your sourcing bridge, we help accelerate assay development from concept to clinic.