Anti-ABCF1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q8NE71

Core Product Specifications and Parameters

Biological Background: ABCF1 Function and Localization

• ABCF1 encodes ATP-binding cassette sub-family F member 1, also known as ABC50 or TNF-alpha-stimulated ABC protein.
• Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation and is not involved in ribosome biogenesis.
• The protein localizes to the cytoplasm, nucleus, nucleoplasm, and nuclear envelope.
• ABCF1 is ubiquitously expressed in human tissues.
• As a member of the ATP-binding cassette family, ABCF1 contains nucleotide-binding domains and is classified as an activator.
• Alternative splicing generates multiple isoforms, and the protein is subject to phosphorylation.
• Structural studies (3D-structure) and proteomic identifications support its functional characterization.

Experimental Guidance and Technical Tips

• For Western blot, the antibody is expected to detect a band at approximately 96 kDa corresponding to ABCF1; include positive and negative controls to confirm specificity.
• In immunohistochemistry (paraffin-embedded sections), optimize antigen retrieval and antibody dilution; human tissue sections with known ubiquitous expression can serve as suitable controls.
• For ELISA applications, the peptide immunogen may be used to validate detection; cross-reactivity with other ABCF family members should be assessed based on sequence homology.
• Since the antibody recognizes human ABCF1, reactivity with orthologs from other species should be experimentally verified if cross-species applications are intended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For ABCF1-focused projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you are developing translation initiation assays or investigating ATP-binding cassette family proteins, our supply chain ensures reliable, high-quality raw materials tailored to your research and diagnostic needs.