Anti-ABCE1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P61221

Core Product Specifications and Parameters

Biological Background: ABCE1 Function and Localization

• ABCE1, also known as RNase L inhibitor (RLI) or 2'-5'-oligoadenylate-binding protein, is a member of the ATP-binding cassette (ABC) transporter family with a unique role in translation and ribosome recycling.
• Functions as a nucleoside-triphosphatase (NTPase) that hydrolyzes ATP, GTP, UTP, and CTP, and facilitates ribosome disassembly by splitting 80S ribosomes into free 60S and 40S subunits. Related references: PMID:20122402 PMID:21448132
• Essential for the No-Go Decay (NGD) pathway, where it is recruited to stalled ribosomes by the Pelota-HBS1L complex and drives disassembly, leading to mRNA degradation. Related references: PMID:21448132
• Acts as a negative regulator of the interferon-regulated 2-5A/RNase L pathway by inhibiting RNASEL activity and antagonizing 2-5A binding, thus modulating antiviral responses. Related references: PMID:9660177 PMID:11585831 PMID:9847332
• Localizes to both the cytoplasm and mitochondria; involved in mitochondrial quality control through PINK1-regulated ubiquitination and mitophagy. Related references: PMID:29861391
• Contains iron-sulfur clusters (4Fe-4S) and ATP-binding domains, and is subject to post-translational modifications including phosphorylation and ubiquitination.
• During HIV-1 infection, may act as a chaperone for capsid assembly, and during EMCV and HIV-1 infections, plays a role in down-regulating the 2-5A/RNASEL pathway.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 500-599 of human ABCE1; the antibody may preferentially recognize a linear epitope, which can be advantageous for Western blotting but may require optimization in immunofluorescence where native conformation is preserved.
• Validated for Western blotting (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA; for best results, titrate the antibody in your specific experimental system and include appropriate controls.
• The target protein has a predicted molecular weight of ~67 kDa; consider using a mid-percentage SDS-PAGE gel and standard PVDF or nitrocellulose membranes for transfer.
• Cross-reactivity with human and mouse ensures suitability for studies in both species, but verify specificity in your model organism through knockdown or knockout controls if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing high-quality monoclonal antibodies like this anti-ABCE1 antibody for research into translation mechanisms and antiviral pathways. We support diagnostic manufacturers with validated antibody pairs, optimized bulk reagents, and rare target sourcing, helping streamline your assay development from concept to clinic. Whether you need ancillary reagents or customized technical consultation, CamelBio bridges your procurement gaps with reliable supply solutions.