Anti-ABCD1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P33897

Core Product Specifications and Parameters

Biological Background: ABCD1 Function and Localization

• ABCD1, also called adrenoleukodystrophy protein (ALDP), is a member of the ATP-binding cassette (ABC) transporter sub-family D, encoded by the ABCD1 (ALD) gene.
• It functions as an ATP-dependent transporter that imports very long chain fatty acyl-CoA (VLCFA-CoA) into peroxisomes, and also possesses fatty acyl-CoA thioesterase (ACOT) activity that hydrolyzes VLCFA-CoA prior to transport, an essential step in the import process. Related references: PMID:11248239, PMID:15682271, PMID:16946495, PMID:29397936, PMID:33500543
• Plays a central role in the degradation and biosynthesis of VLCFAs by regulating peroxisomal beta-oxidation, mitochondrial function, and microsomal fatty acid elongation. Related references: PMID:21145416, PMID:23671276
• Involved in myelination: negatively regulates microsomal fatty acid elongation during active myelination, and is required for axon and myelin maintenance.
• Controls the cellular response to oxidative stress by regulating mitochondrial oxidative phosphorylation and depolarization.
• Modulates the inflammatory response through positive regulation of peroxisomal VLCFA beta-oxidation.
• Subcellular localization includes peroxisomal, mitochondrial, lysosomal, and endoplasmic reticulum membranes, reflecting its multifaceted intracellular transport roles.
• Mutations in ABCD1 cause X-linked adrenoleukodystrophy (ALD), a severe neurodegenerative disorder marked by VLCFA accumulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 340–500) of human ABCD1; validate detection of full-length protein and potential isoforms using appropriate positive controls.
• For Western blot, anticipate a specific band around 83 kDa; use human or mouse cell lines known to express ABCD1 (e.g., fibroblasts, brain tissue) to confirm reactivity.
• For immunofluorescence/ICC, optimize fixation/permeabilization conditions, and consider co-staining with peroxisomal markers to verify subcellular localization.
• ELISA-based detection should employ a recombinant ABCD1 protein standard matching the immunogen sequence for accurate quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials by offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents. This ABCD1 polyclonal antibody supports research into peroxisomal disorders and VLCFA metabolism, and CamelBio can provide bulk-scale production, validated antibody pairs, and customized sourcing solutions to meet your diagnostic development needs. Partner with us to secure reliable raw materials and accelerate your assay pipeline.