Anti-ABCD1 / ALD Rabbit Monoclonal Antibody for WB, ELISA - P33897

Core Product Specifications and Parameters

Biological Background: ABCD1 Function and Localization

• ABCD1, also known as adrenoleukodystrophy protein (ALDP), is an ATP-dependent transporter of the ATP-binding cassette (ABC) family that mediates the transport of very long chain fatty acid (VLCFA)-CoA from the cytosol into the peroxisome lumen (PubMed:11248239, PubMed:15682271, PubMed:16946495). Related references: PMID:11248239, PMID:15682271, PMID:16946495
• The protein also possesses fatty acyl-CoA thioesterase (ACOT) activity, hydrolyzing VLCFA-CoA into free VLCFA prior to ATP-dependent translocation, a step essential for peroxisomal VLCFA import (PubMed:29397936, PubMed:33500543). Related references: PMID:29397936, PMID:33500543
• ABCD1 participates in VLCFA degradation and biosynthesis through peroxisomal β-oxidation, influences mitochondrial function, and regulates microsomal fatty acid elongation (PubMed:21145416, PubMed:23671276). Related references: PMID:21145416, PMID:23671276
• It plays a role in active myelination, axon maintenance, cellular oxidative stress response, and inflammatory signaling (By similarity).
• ABCD1 localizes to multiple organelle membranes, including peroxisome, mitochondrion, lysosome, and endoplasmic reticulum membranes.
• The protein is subject to glycosylation and phosphorylation and contains ATP-binding, nucleotide-binding, and transmembrane domains.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 650–745 of the C-terminal region of human ABCD1, which is expected to be exposed on the cytoplasmic side of peroxisomal and other organelle membranes.
• For Western blotting, a band near 83 kDa is predicted; we recommend using positive control lysates from human or mouse tissues/cell lines known to express ABCD1 (e.g., adrenal gland, brain, or fibroblasts).
• The antibody’s human–mouse cross-reactivity enables its use in heterologous expression or knock-in models; however, expression levels and post‑translational modifications should be verified in the chosen experimental system.
• For ELISA applications, consider titrating the antibody to determine optimal coating and detection conditions; pairing with a validated detection antibody may enhance sensitivity.
• As with any IVD raw material, we advise conducting rigorous in‑house validation under your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for diagnostic manufacturers, laboratories, and research institutes, providing validated monoclonal antibodies, bulk ancillary reagents, and custom raw material sourcing. The ABCD1/ALDP antibody supports research into peroxisomal disorders, adrenoleukodystrophy, and fatty acid metabolism. We also offer technical consultation and customized IVD raw material sourcing to streamline your assay development from concept to clinic.