Anti-ABCC6 Rabbit Polyclonal Antibody for WB, ELISA - O95255

Core Product Specifications and Parameters

Biological Background: ABCC6 Function and Localization

• ABCC6 encodes ATP-binding cassette sub-family C member 6, also known as multidrug resistance-associated protein 6 (MRP6) and multi-specific organic anion transporter E (MOAT-E). It belongs to the ABC transporter family and is implicated in drug resistance and mineralization disorders.
• Functions as an ATP-dependent transporter that actively extrudes physiological compounds and xenobiotics from cells. Mediates the transport of glutathione conjugates like leukotriene C4 (LTC4) and N-ethylmaleimide S-glutathione (NEM-GS), and an anionic cyclopentapeptide endothelin antagonist, BQ-123.
  Related references: PMID:11880368 PMID:12414644
• Plays a pivotal role in calcium homeostasis by mediating the release of nucleoside triphosphates, predominantly ATP, into the circulation. ATP is rapidly converted by ENPP1 to AMP and inorganic pyrophosphate (PPi), a potent inhibitor of mineralization.
• Inhibits TNF-mediated apoptosis by blocking one or more caspases, suggesting a modulatory role in cell survival pathways.
• Subcellular localization includes the basal and basolateral cell membrane, as well as the endoplasmic reticulum membrane, consistent with its transporter and secretory functions.
• Tissue distribution is highest in the kidney and liver, with very low expression in other tissues. In testis, it is found in peritubular myoid cells, Leydig cells, and Sertoli cells, implying a role in blood-testis barrier transport (PubMed:35307651).

Related references: PMID:35307651

• Post-translational modifications likely include phosphorylation and glycosylation (keywords: phosphoprotein, glycoprotein), which may regulate its activity and stability.
• Genetic variants in ABCC6 are associated with pseudoxanthoma elasticum (PXE) and generalized arterial calcification of infancy (GACI2), underscoring its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 830–940 of human ABCC6, a region likely located intracellularly. For western blot, use denaturing and reducing conditions to fully expose the epitope.
• The predicted molecular weight is approximately 165 kDa; however, glycosylation may cause migration at a higher apparent mass. Include appropriate positive controls (e.g., kidney or liver lysates) and negative controls.
• For ELISA, consider using the recombinant immunogen as a standard or coating antigen. Optimize antibody concentration through checkerboard titration.
• Cross-reactivity with mouse and rat is reported; validate performance in your model system, as epitope conservation may vary.
• ABCC6 expression can be low in many cell lines; consider using over-expression systems or tissue extracts for initial validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including this anti-ABCC6 polyclonal antibody for applications in transporter biology and mineralization research. We offer validated antibody pairs, optimized recombinant antigens, and bulk ancillary reagents to streamline assay development. For rare or custom targets like ABCC6, our expert sourcing services ensure rapid access to the materials you need, from concept to clinic.