Anti-ABCC5 Rabbit Polyclonal Antibody for WB, ELISA - O15440

Core Product Specifications and Parameters

Biological Background: ABCC5 Function and Localization

• ABCC5, also known as MRP5 or MOAT-C, is a member of the ATP-binding cassette (ABC) transporter family, specifically the ABCC subfamily.

• Functions as an ATP-dependent efflux pump for physiological compounds, including cyclic nucleotides (cAMP, cGMP), folic acid, and N-lactoyl-amino acids. Related references: PMID:10893247 PMID:12637526 PMID:12695538

• Exhibits low-affinity transport of anticancer drugs (e.g., methotrexate, nucleotide analogs) and antivirals (e.g., PMEA), potentially contributing to drug resistance. Related references: PMID:10840050 PMID:12435799 PMID:12695538

• Acts as a heme transporter, shuttling cytosolic heme to the secretory pathway. Related references: PMID:24836561

• May regulate energy metabolism via modulation of glucagon-like peptide 1 (GLP-1) secretion from enteroendocrine cells (By similarity).

• Localizes to multiple cellular membranes: basolateral and apical cell membranes, Golgi apparatus, endosomes, and cytoplasmic granules.

• Ubiquitously expressed, with highest levels in brain and muscle; predominant isoform in retinal pigment epithelium, bladder, and stomach. Related references: PMID:10438534 PMID:15501592 PMID:9827529 PMID:17521428

• Key structural features include two nucleotide-binding domains, multiple transmembrane helices, and post-translational modifications such as glycosylation and phosphorylation (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-179) of human ABCC5, which is predicted to be intracellular; for native protein detection, permeabilization may be required.
• For Western blotting, a band of approximately 161 kDa is expected; consider using reducing conditions and a high-range molecular weight marker.
• Validate antibody specificity in relevant cell lines or tissues known to express ABCC5 (e.g., brain, muscle, retinal pigment epithelium).
• Optimize primary antibody dilution for your specific assay system, as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing for diagnostic manufacturers and research labs. This ABCC5 polyclonal antibody supports studies on drug resistance, heme transport, and metabolic regulation. Explore our validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.