Anti-ABCB4 Polyclonal Antibody for WB, ELISA - P21439

Core Product Specifications and Parameters

Biological Background: ABCB4 Function and Localization

• ABCB4 functions as an energy-dependent phospholipid floppase that translocates phosphatidylcholine from the inner to the outer leaflet of the canalicular membrane, a critical step for biliary lipid secretion and protection of the biliary tree from bile salt detergent activity. Related references: PMID:17523162, PMID:21820390, PMID:23468132
• The protein is required for proper phospholipid bile formation and indirectly promotes cholesterol efflux from hepatocytes into the canalicular lumen in the presence of bile salts. Related references: PMID:24045840
• ABCB4 promotes biliary phospholipid secretion through vesiculation of the canalicular plasma membrane, releasing phosphatidylcholine into bile. Related references: PMID:28012258, PMID:9366571
• In cooperation with ATP8B1, it protects hepatocytes from the deleterious detergent activity of bile salts. Related references: PMID:21820390
• Subcellularly, ABCB4 localizes to the apical cell membrane, membrane rafts, cytoplasm, and clathrin-coated vesicles, consistent with its role in canalicular transport.
• The protein belongs to the ATP-binding cassette (ABC) transporter family, containing characteristic nucleotide-binding domains and multiple transmembrane helices; it undergoes glycosylation and phosphorylation.
• Mutations in ABCB4 are associated with progressive familial intrahepatic cholestasis type 3 (PFIC-3) and other hepatobiliary disorders, highlighting its essential role in liver function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-110), which may provide specificity for ABCB4 over closely related ABC transporters; cross-reactivity with human and mouse orthologs is confirmed.
• For Western blotting, the predicted molecular weight is approximately 142 kDa; however, post-translational modifications may alter migration. Validate band size in relevant tissue or cell lysates (e.g., liver, hepatocytes).
• In ELISA, consider using the antibody as either a capture or detection reagent, and validate performance with recombinant ABCB4 or native samples; the polyclonal nature may offer broader epitope coverage.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents. This anti-ABCB4 polyclonal antibody is positioned for hepatobiliary disease research, and we can source companion reagents such as matched antigen controls and secondary detection systems. Beyond standard catalog items, CamelBio specializes in custom and bulk sourcing of rare-target raw materials, accelerating your assay development from concept to clinical application.