Anti-ABCA8A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8K442

Core Product Specifications and Parameters

Biological Background: ABCA8A Function and Localization

• ABCA8A is an ATP-binding cassette (ABC) transporter that mediates the efflux of cholesterol and taurocholate, playing a role in cellular lipid homeostasis.
• Through interaction with ABCA1, ABCA8A potentiates cholesterol efflux to lipid-free apolipoprotein A-I (APOA1), thereby regulating high-density lipoprotein (HDL) cholesterol levels.
• ABCA8A belongs to the ABCA subfamily of ABC transporters, which are involved in lipid transport across cellular membranes.
• The protein localizes to the cell membrane, specifically enriched at the basolateral membrane, consistent with its role in directional substrate transport.
• ABCA8A is broadly expressed in tissues, with high expression in liver, heart, and skeletal muscle, and detectable levels in lung and testis. Related references: PMID:12532264 PMID:16445568 PMID:28882873 PMID:29300488
• Post-translational modifications include glycosylation, and the protein contains transmembrane helices, nucleotide-binding domains, and ATP-binding sites, characteristic of functional ABC transporters.
• It is predicted to function as a translocase, driven by ATP hydrolysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 690–828 of mouse ABCA8A; consider this region's intracellular location when selecting applications.
• For Western blot, mouse liver or heart lysates are suitable positive controls; validate cross-reactivity in human and rat samples separately.
• In IHC-P, perform antigen retrieval and confirm staining in known positive tissues such as liver or heart sections.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with another clone can improve sandwich assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers the ABCA8A rabbit polyclonal antibody as part of a comprehensive portfolio supporting lipid metabolism and cardiovascular disease research. Our one-stop IVD raw-material supply chain includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio for seamless access to high-quality reagents that accelerate your diagnostic and research workflows.