Anti-ABCA7 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IZY2

Core Product Specifications and Parameters

Biological Background: ABCA7 Function and Localization

• ABCA7 is a phospholipid-transporting ATPase that catalyzes the translocation of phosphatidylserine and, to a lesser extent, phosphatidylcholine across membranes, playing a critical role in lipid homeostasis. Related references: PMID:24097981
• It functions in apolipoprotein-mediated lipid efflux, binding APOA1 and participating in cholesterol and phospholipid efflux from cells, which is important for reverse cholesterol transport. Related references: PMID:12917409 PMID:14570867 PMID:14592415
• ABCA7 is involved in macrophage-mediated phagocytosis, facilitating the clearance of apoptotic cells and amyloid-beta, and its phagocytic activity is stimulated by APOA1 or APOA2. Related references: PMID:12925201
• The protein regulates cellular ceramide homeostasis, particularly during keratinocyte differentiation, highlighting its role in sphingolipid metabolism. Related references: PMID:12925201
• ABCA7 influences amyloid-beta production and clearance; it limits amyloid-beta generation by regulating amyloid precursor protein (APP) endocytosis/processing and promotes amyloid-beta phagocytosis by microglia and macrophages, linking it to Alzheimer's disease pathology. Related references: PMID:26260791
• Localized to the cell membrane, Golgi apparatus, early endosomes, endoplasmic reticulum, and phagocytic cup, ABCA7's subcellular distribution supports its roles in lipid transport and phagocytosis.
• Two major isoforms exist: isoform 1 is strongly expressed in brain and bone marrow, while isoform 2 is abundant in lymph node, spleen, thymus, and trachea, suggesting tissue-specific functions.
• The protein is widely expressed, with high levels in myelo-lymphatic tissues (peripheral leukocytes, thymus, spleen, bone marrow) and detectable expression in the hippocampus and cerebellum, consistent with its immune and neural roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 2010–2146) of human ABCA7; verify sequence conservation if detecting both isoforms or cross-reacting species.
• For Western blot (WB), note the large predicted molecular weight (~234 kDa); use low-percentage gels, extended transfer times, and high molecular weight markers.
• In immunofluorescence (IF/ICC), expect plasma membrane, Golgi, endosomal, and phagocytic cup staining; co-localization with organelle markers can confirm specificity.
• For ELISA, consider using the antibody as a capture or detection reagent; validate with recombinant ABCA7 protein or lysates from expressing cells.
• When testing mouse or rat samples, confirm cross-reactivity by aligning the immunogen sequence with the respective orthologs; the antibody is reported to recognize both species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, technical services, and consulting—from concept to clinic. For ABCA7, a key target in Alzheimer's disease, lipid metabolism, and immunology, we offer validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents to support your assay development. Contact us to discuss your specific sourcing requirements for rare or specialized targets.