Anti-ABCA4 Rabbit Polyclonal Antibody for WB, ELISA - P78363

Core Product Specifications and Parameters

Biological Background: ABCA4 Function and Localization

• ABCA4 (ATP-binding cassette sub-family A member 4) is also known as RIM ABC transporter, RmP, retinal-specific phospholipid-transporting ATPase, and Stargardt disease protein. Encoded by the ABCA4 gene, it is a transmembrane protein with a molecular weight of approximately 256 kDa.
• Catalyzes the ATP-dependent transport of retinal-phosphatidylethanolamine conjugates (11-cis and all-trans N-retinylidene-PE) from the lumen to the cytoplasmic leaflet of photoreceptor outer segment disk membranes. This flippase activity clears photogenerated all-trans-retinal, preventing accumulation and toxic bisretinoid formation (PubMed:10075733, PubMed:20404325, PubMed:22735453).
  Related references: PMID:10075733, PMID:20404325, PMID:22735453
• Displays intrinsic ATPase activity in vitro, and may exhibit GTPase activity modulated by the lipid environment and retinoid compounds (PubMed:33605212, PubMed:39128720, PubMed:29847635, PubMed:22735453).
  Related references: PMID:33605212, PMID:39128720, PMID:29847635, PMID:22735453
• Localizes to the membrane of photoreceptor outer segment disks, and has also been detected in the endoplasmic reticulum and cytoplasmic vesicles.
• Tissue expression is retinal-specific, predominantly found in the rim region of rod photoreceptor cells.
• Genetic variants in ABCA4 are linked to several inherited retinal dystrophies, including Stargardt disease (STGD1), cone-rod dystrophy (CORD3), retinitis pigmentosa (RP19), and age-related macular degeneration (ARMD2). The protein undergoes post-translational modifications such as glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110–300, an N-terminal region, which may provide reactivity with various ABCA4 isoforms or fragments. Confirm specificity in your model system.
• For Western blotting, note the high molecular weight (~256 kDa). Use a low-percentage SDS-PAGE gel (e.g., 6–8%) and optimize transfer conditions (e.g., overnight wet transfer) for efficient blotting of large proteins.
• For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent. Pair with a matched monoclonal or another polyclonal antibody targeting a distinct epitope for sandwich assays, and validate performance with known positive controls.
• Recommended dilution factors should be determined empirically; literature and general guidelines can serve as starting points.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, laboratories, and research institutes with comprehensive access to high-quality raw materials, including validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents. This ABCA4 Rabbit pAb targets a photoreceptor-specific transporter critical in vision science and retinal disease research, and CamelBio can support your diagnostic or research workflows with consistent supply and technical consultation. Contact us to streamline your sourcing from concept to clinic.