Anti-ABCA2 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q9BZC7

Core Product Specifications and Parameters

Biological Background: ABCA2 Function and Localization

• ABCA2 (ATP-binding cassette sub-family A member 2), also known as ATP-binding cassette transporter 2 or ABC2, is a member of the ABC transporter superfamily involved in ATP-dependent translocation of substrates across membranes.
• It functions as a probable lipid transporter that modulates cholesterol sequestration in the late endosome/lysosome by regulating intracellular sphingolipid metabolism, participating in cholesterol homeostasis. Related references: PMID:15238223, PMID:21810484, PMID:24201375
• ABCA2 may alter the transbilayer distribution of ceramide in intraluminal membrane bilayers, favoring its retention in the outer leaflet and leading to increased acid ceramidase activity, which facilitates ceramide deacylation to sphingosine and lysosomal free cholesterol sequestration. Related references: PMID:24201375
• The protein is also involved in regulating amyloid-beta (Aβ) production, either by modulating sphingolipid metabolism to affect amyloid precursor protein (APP) transcription or through its role in γ-secretase processing of APP. Related references: PMID:22086926, PMID:26510981
• ABCA2 may play a role in myelin formation (by similarity).
• Subcellularly, ABCA2 localizes primarily to the membranes of endosomes and lysosomes.
• Tissue expression analysis shows high levels in brain, peripheral blood leukocytes, and ovary, with lower expression in kidney and liver.
• Post-translational modifications include glycosylation, phosphorylation, and methylation; the protein features two ATP-binding domains and multiple transmembrane helices, consistent with its transporter function.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 140–350) lies within the N-terminal region of human ABCA2; users should verify sequence identity with the target species if cross-reactivity is expected beyond human, mouse, and rat.
• For Western blot, due to the large protein size (~270 kDa), use a high-percentage polyacrylamide gel (e.g., 6–8%) and extended transfer times for efficient blotting.
• In immunofluorescence/immunocytochemistry, fixation and permeabilization protocols should be optimized to detect endosomal/lysosomal membrane proteins; consider using methanol or detergent-based permeabilization.
• ELISA performance can be assessed using recombinant ABCA2 fragments or cell lysates; indirect ELISA may be suitable for titer determination.
• When analyzing mouse or rat tissues, confirm cross-reactivity through sequence alignment of the immunogen region and consider using appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic developers reliable access to anti-ABCA2 antibodies and related IVD raw materials, supporting studies in cholesterol transport, lysosomal biology, and neurodegenerative disease research. Beyond this product, our one-stop platform provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, streamlining your transition from concept to clinic.