Anti-ABCA12 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q86UK0

Core Product Specifications and Parameters

Biological Background: ABCA12 Function and Localization

• ABCA12 encodes a member of the ATP-binding cassette (ABC) transporter superfamily and functions as a glucosylceramide transporter essential for the formation of lipid lamellae in the stratum corneum, critical for skin barrier integrity. Related references: PMID:16007253, PMID:20869849

• It also mediates the transport of lamellar granule-associated proteolytic enzymes, regulating desquamation and keratinocyte differentiation. Related references: PMID:19179616

• In macrophages, ABCA12 contributes to cellular cholesterol homeostasis by regulating ABCA1-dependent cholesterol efflux. (By similarity)

• The protein localizes to the membrane of cytoplasmic secretory vesicles and the Golgi apparatus in keratinocytes.

• ABCA12 is expressed in various tissues including stomach, placenta, testis, fetal brain, and prominently in the granular layers of the epidermis and pancreatic islets. Related references: PMID:12697999, PMID:16007253, PMID:17591952, PMID:17927575, PMID:19179616, PMID:32072744

• Mutations in ABCA12 are associated with autosomal recessive congenital ichthyosis, including harlequin ichthyosis and lamellar ichthyosis.

• Post-translational modifications include glycosylation and nucleotide binding; the protein contains transmembrane domains and is involved in lipid transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 2456–2595), which may confer isoform specificity; validate in ABCA12-expressing cell lines or tissues such as skin keratinocytes or pancreatic islets.
• For immunofluorescence applications, consider using cultured epidermal keratinocytes or skin tissue sections, focusing on staining in the granular layer where ABCA12 is abundant.
• ELISA can be used for quantitative detection; use appropriate recombinant protein standards as positive controls and include negative controls to assess signal specificity.
• Pre-absorption of the antibody with the immunogen peptide can help confirm specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive one-stop sourcing platform for IVD raw materials, supporting your research with high-quality ABCA12 antibodies for lipid transport and dermatology studies. We provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Leverage our expertise to access rare target raw materials and streamline your workflow from discovery to diagnostics.