Anti-ABCA1 Rabbit Polyclonal Antibody - O95477

Core Product Specifications and Parameters

Biological Background: ABCA1 Function and Localization

• Encodes phospholipid-transporting ATPase ABCA1, a member of the ATP-binding cassette (ABC) transporter family, involved in translocating phosphatidylcholine and phosphatidylserine from the cytoplasmic to the extracellular leaflet of the membrane coupled to ATP hydrolysis. Related references: PMID:24097981 PMID:35974019

• Plays a key role in cholesterol efflux from cells to apolipoproteins, facilitating the formation of nascent high-density lipoproteins (HDLs) and reverse cholesterol transport. Related references: PMID:10533863 PMID:14754908 PMID:24097981

• Localizes to the cell membrane and endosomes, consistent with its role in lipid trafficking between intracellular compartments and the plasma membrane.

• Widely expressed, with highest levels in macrophages, reflecting its critical function in macrophage cholesterol homeostasis and atherosclerosis prevention.

• Post-translational modifications include phosphorylation and palmitoylation, which may regulate its activity and membrane association.

• Clinically significant, with disease variants in ABCA1 linked to Tangier disease and familial HDL deficiency, characterized by severely reduced HDL cholesterol and increased cardiovascular risk.

• The protein contains two ATP-binding domains, disulfide bonds, and glycosylation sites, and is predicted to span the membrane multiple times.

Experimental Guidance and Technical Tips

• Given the immunogen corresponds to the C-terminal region (aa 1870–2120) of human ABCA1, the antibody may recognize epitopes in this distal domain, which is predicted to be extracellular; thus, it may be suitable for detecting surface-localized ABCA1 in IF/ICC under non-permeabilizing conditions. However, validate in the relevant sample system.
• Due to the large molecular weight of ABCA1 (~254 kDa), consider extended transfer times or low-percentage gels (e.g., 6–8% SDS-PAGE) and verify transfer efficiency with a protein ladder or total protein stain.
• For WB, a loading control of similar high molecular weight may be necessary; alternatively, Ponceau S staining can confirm transfer.
• Cross-reactivity with mouse and rat has been reported; however, the degree of reactivity in these species should be empirically determined using tissue or cell lysates expressing ABCA1.

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