Anti-ABCA1 Polyclonal Antibody for WB, IF/ICC, ELISA - O95477

Core Product Specifications and Parameters

Biological Background: ABCA1 Function and Localization

• Phospholipid-transporting ATPase ABCA1, also known as ATP-binding cassette sub-family A member 1, ABC-1, or cholesterol efflux regulatory protein.
• Functions in ATP-dependent translocation of phosphatidylcholine from the cytoplasmic to the extracellular membrane leaflet, critical for phospholipid transfer to apolipoproteins and HDL formation (PubMed:24097981, PubMed:35974019, PubMed:14754908). Related references: PMID:24097981, PMID:35974019, PMID:14754908
• Also facilitates intracellular cholesterol efflux to apolipoproteins, contributing to nascent HDL biogenesis (PubMed:10533863). Related references: PMID:10533863
• Subcellularly localizes to the cell membrane and endosomes.
• Widely expressed with highest abundance in macrophages.
• Belongs to the ABC transporter family; classified under keywords including ATP-binding, cholesterol metabolism, disulfide bond, glycoprotein, and transmembrane transport.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 1170-1350) of human ABCA1, which may provide specificity for full-length protein detection.
• For Western blot, consider using optimized extraction methods for large (~254 kDa) multi-pass transmembrane proteins, and validate in macrophage cell lines known to express ABCA1.
• For immunofluorescence (IF/ICC), ensure proper permeabilization to detect membrane‑localized epitopes, and confirm staining patterns against known localization.
• The antibody is reported to cross‑react with mouse samples; validate in the relevant animal model as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for rare targets like ABCA1. This polyclonal antibody facilitates research into cholesterol metabolism, macrophage‑driven HDL formation, and atherosclerosis. Contact our team for tailored raw‑material sourcing and technical assistance from development to clinical translation.