Anti-AARS/AARS1 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P49588

Core Product Specifications and Parameters

Biological Background: Alanine--tRNA ligase, cytoplasmic Function and Localization

• Alanine--tRNA ligase, cytoplasmic (AARS1/AARS), also known as alanyl-tRNA synthetase (AlaRS), is a multifunctional enzyme encoded by the AARS1 gene.
• Its canonical function is to catalyze the ATP-dependent attachment of alanine to its cognate tRNA(Ala), a step essential for protein translation. It also possesses an editing domain to hydrolyze mischarged tRNA(Ala). Related references: PMID:27622773, PMID:27911835, PMID:28493438
• Under high lactate conditions, AARS1 moonlight as a protein lactyltransferase, transferring lactyl groups to lysine residues of target proteins. This non-canonical activity regulates transcription factors such as TP53/p53, TEAD1, and YAP1. Related references: PMID:38512451, PMID:38653238, PMID:39322678
• The protein localizes to both the cytoplasm and nucleus, consistent with its roles in translation and transcriptional regulation.
• Post-translational modifications of AARS1 include acetylation, phosphorylation, methylation, and ubiquitination, which may modulate its enzymatic activities and interactions.
• Mutations in AARS1 are associated with a spectrum of neurological disorders, including Charcot-Marie-Tooth disease type 2N (CMT2N), developmental and epileptic encephalopathy 29 (DEE29), and hypomyelinating leukodystrophy (HDLS2), highlighting its importance in neuronal homeostasis.
• It requires zinc and ATP as cofactors for its aminoacylation and editing functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 at the N-terminus of human AARS. This region may be subject to post-translational modifications; verify detection of modified forms in your experimental system.
• For Western blotting, the expected molecular weight is ~107 kDa. Endogenous AARS1 is ubiquitously expressed; ensure sufficient loading for tissues with lower expression.
• In immunocytochemistry/immunofluorescence (ICC/IF), paraformaldehyde fixation is typically compatible. Since AARS1 shuttles between the nucleus and cytoplasm, cellular localization may vary depending on metabolic state (e.g., lactate levels).
• For immunohistochemistry (IHC), antigen retrieval conditions should be optimized due to the N-terminal epitope. Consider using a citrate-based buffer at pH 6.0.
• When using for ELISA, pair with a validated capture or detection antibody to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with premium IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-AARS polyclonal antibody supports studies on aminoacyl-tRNA synthetases, protein lactylation, and neurological disease mechanisms. For specialized needs—such as rare-target sourcing or custom development—our team provides seamless procurement support from concept to clinic.