Anti-AARS2 Rabbit Polyclonal Antibody for WB and ELISA - Q5JTZ9

Core Product Specifications and Parameters

Biological Background: AARS2 Function and Localization

• AARS2 functions as a mitochondrial alanyl-tRNA synthetase, catalyzing alanine attachment to tRNA(Ala) and editing mischarged tRNA via its editing domain (PubMed:21549344). Related references: PMID:21549344
• Under high lactate conditions, AARS2 acts as a protein lactyltransferase, mediating lysine lactylation of target proteins such as CGAS (PubMed:39322678). Related references: PMID:39322678
• Lactylation of CGAS by AARS2 inhibits cGAS/STING signaling by preventing the formation of liquid-like droplets required for CGAS activation (PubMed:39322678). Related references: PMID:39322678
• It is localized to the mitochondrion, consistent with its role in mitochondrial protein synthesis.
• Mutations in AARS2 are associated with human diseases including cardiomyopathy, neurodegeneration, and primary mitochondrial disorders.
• The protein contains conserved domains for ATP binding, tRNA binding, and zinc coordination, essential for its enzymatic activities.

Experimental Guidance and Technical Tips

• The immunogen spans residues 491–620; for Western blot, consider validating antibody specificity using AARS2 knockout or knockdown samples.
• For ELISA, recommend titrating the antibody with recombinant AARS2 protein or peptide to determine optimal coating concentrations.
• Expected molecular weight ~107 kDa; use appropriate gel percentage (e.g., 6-8%) for clear separation.
• Since it is a polyclonal antibody, lot-to-lot consistency should be verified in the relevant sample system.
• Cross-reactivity with human and mouse makes it suitable for translational research; validate in both species if used interchangeably.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For mitochondrial tRNA synthetase research, including AARS2 and related targets, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare targets, ensuring comprehensive support from concept to clinic.