Anti-AARS2 Polyclonal Antibody for WB, ELISA - Q5JTZ9

Core Product Specifications and Parameters

Biological Background: AARS2 Function and Localization

• AARS2 encodes the mitochondrial alanyl-tRNA synthetase (AlaRS), which catalyzes the attachment of alanine to its cognate tRNA(Ala) during mitochondrial protein synthesis.
• The enzyme also possesses an editing domain that hydrolyzes mischarged tRNA(Ala), ensuring translational fidelity (PubMed:21549344). Related references: PMID:21549344
• Beyond its canonical role, AARS2 functions as a protein lactyltransferase under high lactate conditions, mediating lysine lactylation on target proteins such as CGAS (PubMed:39322678). Related references: PMID:39322678
• AARS2 is localized to the mitochondrion, consistent with its primary function in organellar translation.
• Mutations in AARS2 are associated with human diseases including cardiomyopathy, neurodegeneration, and premature ovarian failure (Keywords: Disease variant).
• The protein contains conserved motifs for ATP binding, zinc coordination, and tRNA recognition (Keywords: ATP-binding, Metal-binding, Zinc, tRNA-binding).
• Alternative names include AARSL, MTALARS, and COXPD8, reflecting its mitochondrial localization and disease associations.
• AARS2 acts as an inhibitor of cGAS/STING innate immune signaling by lactylating CGAS and preventing its phase separation (PubMed:39322678). Related references: PMID:39322678

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 841-985 near the C-terminal region of human AARS2; therefore, the polyclonal antibody may recognize full-length endogenous protein and potentially truncation variants.
• Recommended applications include Western blotting (WB) and ELISA, as supported by the supplier; optimal dilutions should be determined experimentally.
• For WB, consider using mitochondrial-enriched lysates to enhance detection, given the mitochondrial localization of AARS2.
• Due to the high molecular weight (~107 kDa), ensure proper SDS-PAGE conditions and transfer protocols tailored for large proteins.
• Validate antibody specificity in the relevant human cell lines or tissue samples, and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio offers research and diagnostic communities streamlined access to validated antibodies like this anti-AARS2 polyclonal antibody. We support mitochondrial disease and protein modification studies by supplying optimized antibody reagents, ancillary consumables, and technical guidance. From concept to clinic, our one-stop platform bridges your procurement needs with reliable, high-quality materials.